| Literature DB >> 27573410 |
Nadia Neyazi1,2, Taiebeh Mohammadi Farsani1, Zahra Nouri1, Mohammad Hossein Ghahremani3,4, Mohammad Reza Khorramizadeh1,5, Roksana Tajerian6, Elahe Motevaseli7,8.
Abstract
Type 2 diabetes (T2D) is a condition with insufficient insulin production or in the setting of insulin resistance with many origins including intestinal microbiota-related molecular mechanism. Insulin-degrading enzyme (IDE) is responsible for insulin breakdown in various tissues and is known as a potential drug target for T2D. Here, we assessed the effects of cell-free supernatant (CFS) and UV-killed Lactobacillus casei IBRC_M10711 on IDE expression, IDE activity, and insulin degradation in Caco-2 cell line. It was found that CFS and UV-killed L. casei IBRC_M10711 led to lower expression of IDE. UV-killed L. casei IBRC_M10711 significantly inhibited IDE activity but CFS did not. Insulin degradation was affected with none of them. In conclusion, L. casei IBRC_M10711 is effective on IDE expression and its activity, but not on insulin degradation. Future studies are recommended to explore the effect of this probiotic on other substrates of IDE.Entities:
Keywords: Cell-free supernatant; Insulin degradation; Insulin-degrading enzyme; Lactobacillus casei IBRC_M10711; UV-killed
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Year: 2016 PMID： 27573410 DOI： 10.1007/s11626-016-0083-4
Source DB: PubMed Journal: In Vitro Cell Dev Biol Anim ISSN： 1071-2690 Impact factor: 2.416