Literature DB >> 2757190

A least-squares computer method for the determination of the molecular ratio of conjugates between two different proteins from the results of the amino acid analysis.

G Antoni1, R Presentini.   

Abstract

A method for the determination of the composition of a conjugate between two different proteins by amino acid analysis followed by least-squares evaluation of the concentration ratio of the two components is presented. The method is based solely on calculations and avoids the use of labeled residues. A computer program, written in BASIC, is also presented to perform the calculations.

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Year:  1989        PMID： 2757190     DOI： 10.1016/0003-2697(89)90217-0

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  3 in total

1.  Identification of T cell epitopes within a 23-kD antigen (P24) of Toxoplasma gondii.

Authors:  V Duquesne; C Auriault; H Gras-Masse; C Boutillon; F Darcy; M F Cesbron-Delauw; A Tartar; A Capron
Journal:  Clin Exp Immunol       Date:  1991-06       Impact factor: 4.330

2.  Determination of protein concentration for protein-protein conjugates using ultraviolet absorption.

Authors:  Daming Zhu; Feng Qian; Yimin Wu; David S Jones; Christopher Rowe; David L Narum; Patrick Duffy; Louis H Miller; Allan Saul
Journal:  J Immunol Methods       Date:  2012-10-23       Impact factor: 2.303

3.  Biochemical characterization of the molecular interaction between recombinant basic fibroblast growth factor and a recombinant soluble fibroblast growth factor receptor.

Authors:  P Caccia; O Cletini; A Isacchi; L Bergonzoni; G Orsini
Journal:  Biochem J       Date:  1993-09-15       Impact factor: 3.857
  3 in total

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