Parunya Chaiyawat1, Churat Weeraphan2, Pukkavadee Netsirisawan2, Daranee Chokchaichamnankit2, Chantragan Srisomsap3, Jisnuson Svasti3, Voraratt Champattanachai4. 1. Applied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok, Thailand. 2. Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, Thailand. 3. Applied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok, Thailand Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, Thailand. 4. Applied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok, Thailand Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, Thailand voraratt@cri.or.th.
Abstract
BACKGROUND: O-GlcNAcylation is a single sugar attachment of serine and/or threonine residues on intracellular proteins. Recent reports reveal that it can modify several secretory proteins; however, the underlying mechanisms are largely unexplored. MATERIALS AND METHODS: To investigate whether extracellular vesicles (EVs) carry secretory O-GlcNAc-modified proteins that were isolated from colorectal cancer (CRC) cells, two-dimensional gel electrophoresis followed with O-GlcNAc immunoblotting and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied. RESULTS: It was revealed that the O-GlcNAc modification of many EV proteins was increased in metastatic cells. Among these, transitional endoplasmic reticulum ATPase (TER ATPase) and RuVB-like1 were successfully confirmed for the O-GlcNAc modification in which the levels were significantly higher in EVs of metastatic CRC cell line. CONCLUSION: These data, demonstrate that proteins carried by EVs are O-GlcNAc-modified. Importantly, elevated aberrant O-GlcNAcylation of EV proteins might serve as a potential biomarker of metastatic CRC. Copyright
BACKGROUND: O-GlcNAcylation is a single sugar attachment of serine and/or threonine residues on intracellular proteins. Recent reports reveal that it can modify several secretory proteins; however, the underlying mechanisms are largely unexplored. MATERIALS AND METHODS: To investigate whether extracellular vesicles (EVs) carry secretory O-GlcNAc-modified proteins that were isolated from colorectal cancer (CRC) cells, two-dimensional gel electrophoresis followed with O-GlcNAc immunoblotting and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied. RESULTS: It was revealed that the O-GlcNAc modification of many EV proteins was increased in metastatic cells. Among these, transitional endoplasmic reticulum ATPase (TER ATPase) and RuVB-like1 were successfully confirmed for the O-GlcNAc modification in which the levels were significantly higher in EVs of metastatic CRC cell line. CONCLUSION: These data, demonstrate that proteins carried by EVs are O-GlcNAc-modified. Importantly, elevated aberrant O-GlcNAcylation of EV proteins might serve as a potential biomarker of metastatic CRC. Copyright
Authors: Elham Hosseini-Beheshti; Steven Pham; Hans Adomat; Na Li; Emma S Tomlinson Guns Journal: Mol Cell Proteomics Date: 2012-06-21 Impact factor: 5.911
Authors: Jacques Ferlay; Isabelle Soerjomataram; Rajesh Dikshit; Sultan Eser; Colin Mathers; Marise Rebelo; Donald Maxwell Parkin; David Forman; Freddie Bray Journal: Int J Cancer Date: 2014-10-09 Impact factor: 7.396
Authors: Mohamed I Gatie; Danielle M Spice; Amritpal Garha; Adam McTague; Mariam Ahmer; Alexander V Timoshenko; Gregory M Kelly Journal: Biomolecules Date: 2022-04-22