Nu T Lu1, Natalie M Liu2, James Q Vu2, Darshil Patel2, Whitaker Cohn3, Joe Capri4, Mary Ziegler2, Nikita Patel2, Angela Tramontano5, Roger Williams6, Julian Whitelegge3, Samuel W French7. 1. Department of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, CA, U.S.A Department of Hematology and Oncology, University of California Los Angeles, Los Angeles, CA, U.S.A. 2. Department of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, CA, U.S.A. 3. Department of Psychiatry and Biobehavioral Sciences, University of California Los Angeles, Los Angeles, CA, U.S.A. 4. Department of Psychiatry and Biobehavioral Sciences, University of California Los Angeles, Los Angeles, CA, U.S.A Department of Molecular and Medical Pharmacology, University of California Los Angeles, Los Angeles CA, U.S.A. 5. Institute for Technology Assessment, Massachusetts General Hospital, Boston, MA, U.S.A. 6. The Foundation for Liver Research-The Institute for Hepatology, London, U.K. 7. Department of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, CA, U.S.A sfrench@mednet.ucla.edu.
Abstract
BACKGROUND: Patients with chronic hepatitis C virus (HCV) infection are at risk of serious complications of cirrhosis and hepatocellular carcinoma (HCC). Mass spectrometry (MS) is a versatile methodology that produces a global proteomic landscape for analysis of cancer mechanisms. MATERIALS AND METHODS: Using multiplex peptide stable isotopic labeling and immobilized metal affinity chromatography (IMAC), we enriched and quantified the phosphoproteome of HCC, with and without HCV. While raw data identified protein targets based on expression alone, we also used abundance groups for comprehensive functional analysis. RESULTS: Analysis of functional differences highlighted deregulated phosphoprotein networks. This uncovered additional candidates that could be directly derived from the MS data. Cellular processes and pathways that may differ with HCV infection include: cytoskeletal dynamics, insulin response, gene expression, and PI3K/AKT oncogenesis. CONCLUSION: This function-focused workflow provides a simple framework to analyze MS data. Phosphoproteome quantitation with inclusive functional analysis can generate hypotheses for liver cancer research to improve early screening and identification of molecular targets for therapy. Copyright
BACKGROUND:Patients with chronic hepatitis C virus (HCV) infection are at risk of serious complications of cirrhosis and hepatocellular carcinoma (HCC). Mass spectrometry (MS) is a versatile methodology that produces a global proteomic landscape for analysis of cancer mechanisms. MATERIALS AND METHODS: Using multiplex peptide stable isotopic labeling and immobilized metal affinity chromatography (IMAC), we enriched and quantified the phosphoproteome of HCC, with and without HCV. While raw data identified protein targets based on expression alone, we also used abundance groups for comprehensive functional analysis. RESULTS: Analysis of functional differences highlighted deregulated phosphoprotein networks. This uncovered additional candidates that could be directly derived from the MS data. Cellular processes and pathways that may differ with HCV infection include: cytoskeletal dynamics, insulin response, gene expression, and PI3K/AKT oncogenesis. CONCLUSION: This function-focused workflow provides a simple framework to analyze MS data. Phosphoproteome quantitation with inclusive functional analysis can generate hypotheses for liver cancer research to improve early screening and identification of molecular targets for therapy. Copyright
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