Balázs Németh1, István Kiss2, Iván Péter3, Zénó Ajtay4, Ádám Németh5, László Márk6, Attila Csorba7, Tamás Kőszegi8, Diána Mühl9, Péter Kustán10. 1. Department of Public Health Medicine, Medical School, University of Pécs, Pécs, Hungary Zsigmondy Vilmos SPA Hospital, Harkány, Hungary balazs.nemeth@aok.pte.hu. 2. Department of Public Health Medicine, Medical School, University of Pécs, Pécs, Hungary. 3. Zsigmondy Vilmos SPA Hospital, Harkány, Hungary. 4. Zsigmondy Vilmos SPA Hospital, Harkány, Hungary Heart Institute, Medical School, University of Pécs, Pécs, Hungary. 5. Heart Institute, Medical School, University of Pécs, Pécs, Hungary. 6. Department of Biochemistry and Medical Chemistry, Medical School, University of Pécs, Pécs, Hungary. 7. Department of Biochemistry and Medical Chemistry, Medical School, University of Pécs, Pécs, Hungary Department of Pharmacognosy, Faculty of Pharmacy, University of Szeged, Szeged, Hungary. 8. Department of Laboratory Medicine, Medical School, University of Pécs, Pécs, Hungary János Szentágothai Research Centre, University of Pécs, Pécs, Hungary. 9. Department of Anaesthesiology and Intensive Therapy, Medical School, University of Pécs, Pécs, Hungary. 10. Department of Laboratory Medicine, Medical School, University of Pécs, Pécs, Hungary János Szentágothai Research Centre, University of Pécs, Pécs, Hungary Department of Anaesthesiology and Intensive Therapy, Medical School, University of Pécs, Pécs, Hungary.
Abstract
BACKGROUND/AIM: Nitric oxide (NO) pathway plays a major role in the development and advancement of inflammation. We aimed to design a study and investigate its feasibility to show the changes of L-arginine, asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA), which are important regulators of the NO pathway. PATIENTS AND METHODS: Concentrations of L-arginine, ADMA and SDMA were measured by liquid chromatography-tandem mass spectrometry. Seventeen septic survival patients were enrolled and blood samples were obtained on the first, third and fifth day after the diagnosis of sepsis. Sixteen non-septic matched controls were recruited. RESULTS: ADMA levels on admission correlated well with sequential organ failure assessment (SOFA) score. During the follow-up, L-arginine/ADMA ratio increased significantly from day 1 to day 3 (p=0.005), then decreased from day 3 to day 5 (p=0.023). CONCLUSION: This study design seems feasible to investigate changes of L-Arginine, ADMA and SDMA in sepsis survival patients.
BACKGROUND/AIM: Nitric oxide (NO) pathway plays a major role in the development and advancement of inflammation. We aimed to design a study and investigate its feasibility to show the changes of L-arginine, asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA), which are important regulators of the NO pathway. PATIENTS AND METHODS: Concentrations of L-arginine, ADMA and SDMA were measured by liquid chromatography-tandem mass spectrometry. Seventeen septic survival patients were enrolled and blood samples were obtained on the first, third and fifth day after the diagnosis of sepsis. Sixteen non-septic matched controls were recruited. RESULTS:ADMA levels on admission correlated well with sequential organ failure assessment (SOFA) score. During the follow-up, L-arginine/ADMA ratio increased significantly from day 1 to day 3 (p=0.005), then decreased from day 3 to day 5 (p=0.023). CONCLUSION: This study design seems feasible to investigate changes of L-Arginine, ADMA and SDMA in sepsis survival patients.
Authors: Balazs Nemeth; Istvan Kiss; Timea Jencsik; Ivan Peter; Zita Kreska; Tamas Koszegi; Attila Miseta; Peter Kustan; Imre Boncz; Andrea Laczo; Zeno Ajtay Journal: In Vivo Date: 2017 May-Jun Impact factor: 2.155