Laura Kiscsatári1, Márta Sárközy2, Bence Kővári3, Zoltán Varga1, Kamilla Gömöri2, Nikolett Morvay4, István Leprán4, Hargita Hegyesi5, Gabriella Fábián1, Bálint Cserni6, Gábor Cserni7, Tamás Csont2, Zsuzsanna Kahán8. 1. Department of Oncotherapy, University of Szeged, Szeged, Hungary. 2. Metabolic Diseases and Cell Signaling Group, Department of Biochemistry, University of Szeged, Szeged, Hungary. 3. Department of Pathology, University of Szeged, Szeged, Hungary. 4. Department of Pharmacology and Pharmacotherapy, University of Szeged, Szeged, Hungary. 5. Department of Morphology and Physiology, College of Health Care, Semmelweis University, Budapest, Hungary. 6. Faculty of Science and Informatics, University of Szeged, Szeged, Hungary. 7. Department of Pathology, University of Szeged, Szeged, Hungary Department of Pathology, Bács-Kiskun County Teaching Hospital, Kecskemét, Hungary. 8. Department of Oncotherapy, University of Szeged, Szeged, Hungary kahan.zsuzsanna@med.u-szeged.hu.
Abstract
BACKGROUND/AIM: Radiation-induced heart disease (RIHD) is a concern during radiotherapy. For its comprehensive study, an in vivo selective heart irradiation model was developed. MATERIALS AND METHODS: Sprague-Dawley rats were irradiated with 50 Gy and functional imaging, biochemical (circulating growth differentiation factor-15 (GDF-15), transforming growth factor-beta (TGF-beta) and morphological (picrosirius red staining of the heart) objectives were tested. RESULTS: Signs and symptoms of RIHD occurred >12 weeks after irradiation with tachypnea, systolic and diastolic dysfunction, cardiac hypertrophy and body development retardation. Plasma GDF-15 was increased 3, 12 and 26, while plasma TGF-beta was increased 12 weeks after irradiation. At autopsy, extensive pleural fluid was found in the irradiated animals. Interstitial fibrosis could be reliably detected and quantified in irradiated hearts after a follow-up time of 19 weeks. CONCLUSION: The studied parameters could be used in future experiments for testing protective agents for prevention of radiation heart injury.
BACKGROUND/AIM: Radiation-induced heart disease (RIHD) is a concern during radiotherapy. For its comprehensive study, an in vivo selective heart irradiation model was developed. MATERIALS AND METHODS:Sprague-Dawley rats were irradiated with 50 Gy and functional imaging, biochemical (circulating growth differentiation factor-15 (GDF-15), transforming growth factor-beta (TGF-beta) and morphological (picrosirius red staining of the heart) objectives were tested. RESULTS: Signs and symptoms of RIHD occurred >12 weeks after irradiation with tachypnea, systolic and diastolic dysfunction, cardiac hypertrophy and body development retardation. Plasma GDF-15 was increased 3, 12 and 26, while plasma TGF-beta was increased 12 weeks after irradiation. At autopsy, extensive pleural fluid was found in the irradiated animals. Interstitial fibrosis could be reliably detected and quantified in irradiated hearts after a follow-up time of 19 weeks. CONCLUSION: The studied parameters could be used in future experiments for testing protective agents for prevention of radiation heart injury.
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