Anne Caspar1, Jörg Mostertz2, Merle Leymann1, Patrick Ziegler3, Katja Evert4, Matthias Evert4, Uwe Zimmermann1, Lars-Ove Brandenburg5, Martin Burchardt1, Matthias B Stope6. 1. Department of Urology, University Medicine Greifswald, Greifswald, Germany. 2. Competence Center Functional Genomics, Junior Research Group Pathoproteomics, University of Greifswald, Greifswald, Germany. 3. Institute of Occupational and Social Medicine, RWTH Aachen Universtity, Aachen, Germany. 4. Department of Pathology, University Regensburg, Regensburg, Germany Department of Pathology, University Medicine Greifswald, Greifswald, Germany. 5. Department of Anatomy and Cell Biology, RWTH Aachen Universtity, Aachen, Germany. 6. Department of Urology, University Medicine Greifswald, Greifswald, Germany matthias.stope@uni-greifswald.de.
Abstract
BACKGROUND/AIM: The high variability of primary cells propagated in vitro led us to study the expression patterns of 11 most commonly accepted and widely used biomarkers specific for prostate cancer (PC) cells in primary cell models. MATERIALS AND METHODS: Primary PC cells from five PC patients were partially subjected to RNA preparation immediately and remaining cells were propagated up to 84 days followed by RNA preparation. Subsequently, biomarker mRNA quantification was performed by quantitative reverse transcription-polymerase chain reaction (RT-PCR) and biomarker transcript concentrations before and after cultivation of primary PC cells were compared. RESULTS: Evaluation of androgen receptor, prostate-specific antigen, acid phosphatase, prostate-specific membrane antigen, fatty acid synthase, cytokeratin types 5/8/19, E-cadherin, epithelial cell adhesion molecule and fibroblast-specific protein 1 demonstrated temporal changes, as well as individual differences in expression, during primary PC cell propagation. CONCLUSION: Experimental design, as well as data evaluation, may need to take under consideration the high variability of biomarker expression in primary PC cells.
BACKGROUND/AIM: The high variability of primary cells propagated in vitro led us to study the expression patterns of 11 most commonly accepted and widely used biomarkers specific for prostate cancer (PC) cells in primary cell models. MATERIALS AND METHODS: Primary PC cells from five PC patients were partially subjected to RNA preparation immediately and remaining cells were propagated up to 84 days followed by RNA preparation. Subsequently, biomarker mRNA quantification was performed by quantitative reverse transcription-polymerase chain reaction (RT-PCR) and biomarker transcript concentrations before and after cultivation of primary PC cells were compared. RESULTS: Evaluation of androgen receptor, prostate-specific antigen, acid phosphatase, prostate-specific membrane antigen, fatty acid synthase, cytokeratin types 5/8/19, E-cadherin, epithelial cell adhesion molecule and fibroblast-specific protein 1 demonstrated temporal changes, as well as individual differences in expression, during primary PC cell propagation. CONCLUSION: Experimental design, as well as data evaluation, may need to take under consideration the high variability of biomarker expression in primary PC cells.
Authors: Leanne Woods-Burnham; Anamika Basu; Christina K Cajigas-Du Ross; Arthur Love; Clayton Yates; Marino De Leon; Sourav Roy; Carlos A Casiano Journal: Prostate Date: 2017-10-14 Impact factor: 4.012
Authors: Erika Heninger; David Kosoff; Tamara S Rodems; Nan Sethakorn; Anupama Singh; Harshitha Gungurthi; Kristin N Carlson; Bing Yang; Cole Gilsdorf; Cheri A Pasch; Dustin A Deming; Leigh Ellis; David J Beebe; David F Jarrard; Joshua M Lang Journal: Med Oncol Date: 2021-09-28 Impact factor: 3.738
Authors: Sharon Yunger; Assaf Bar El; Li-At Zeltzer; Eddie Fridman; Gil Raviv; Menachem Laufer; Jacob Schachter; Gal Markel; Orit Itzhaki; Michal J Besser Journal: Oncoimmunology Date: 2019-10-11 Impact factor: 8.110