Qian Zhang1, Bin Chen1, Dinggui Zhu1, Fuhua Yan2. 1. Nanjing Stomatological Hospital, Medical School of Nanjing University, 30 Zhongyang Road, Nanjing, Jiangsu 210008, China. 2. Nanjing Stomatological Hospital, Medical School of Nanjing University, 30 Zhongyang Road, Nanjing, Jiangsu 210008, China. Electronic address: yanfh@nju.edu.cn.
Abstract
OBJECTIVE: To compare five biomarker levels in gingival crevicular fluid (GCF) in different tooth-sites of subjects with healthy periodontium, aggressive periodontitis and severe chronic periodontitis, and to evaluate the value of these biomarker levels for diagnosis of the type and activity of periodontitis. MATERIALS AND METHODS: Prior to therapy, GCF samples were collected using filter paper strip at different tooth-sites of 10 subjects with healthy periodontium (H), 15 with severe chronic periodontitis (CP) and 15 with aggressive periodontitis (AgP). The strips were weighed and the periodontal clinical parameters were recorded. Levels of interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor-alpha (TNF-α), C-reactive protein (CRP), and alkaline phosphatase (ALP) in GCF were assessed by enzyme-linked immunosorbent assay (ELISA). RESULTS: The volumes of the GCF samples obtained in CP and AgP subjects were significantly higher than those from subjects with healthy periodontium (P<0.05). Levels of IL-6, TNF-α, CRP and ALP were significantly higher in the untreated disease sites in the CP and AgP groups compared to those in control sites in the H group, while IL-10 levels were lower in the CP and AgP groups than those in the control sites in the H group. However, the levels of all five biomarker levels showed significant correlation with the clinical parameters. CONCLUSION: The measurement of five biomarker levels in GCF may facilitate overall screening of periodontitis patients in epidemiological studies and allow estimation of periodontitis activity.
OBJECTIVE: To compare five biomarker levels in gingival crevicular fluid (GCF) in different tooth-sites of subjects with healthy periodontium, aggressive periodontitis and severe chronic periodontitis, and to evaluate the value of these biomarker levels for diagnosis of the type and activity of periodontitis. MATERIALS AND METHODS: Prior to therapy, GCF samples were collected using filter paper strip at different tooth-sites of 10 subjects with healthy periodontium (H), 15 with severe chronic periodontitis (CP) and 15 with aggressive periodontitis (AgP). The strips were weighed and the periodontal clinical parameters were recorded. Levels of interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor-alpha (TNF-α), C-reactive protein (CRP), and alkaline phosphatase (ALP) in GCF were assessed by enzyme-linked immunosorbent assay (ELISA). RESULTS: The volumes of the GCF samples obtained in CP and AgP subjects were significantly higher than those from subjects with healthy periodontium (P<0.05). Levels of IL-6, TNF-α, CRP and ALP were significantly higher in the untreated disease sites in the CP and AgP groups compared to those in control sites in the H group, while IL-10 levels were lower in the CP and AgP groups than those in the control sites in the H group. However, the levels of all five biomarker levels showed significant correlation with the clinical parameters. CONCLUSION: The measurement of five biomarker levels in GCF may facilitate overall screening of periodontitispatients in epidemiological studies and allow estimation of periodontitis activity.
Authors: Kelly R V Villafuerte; Felipe T Dantas; Mario Taba; Michel Messora; Francisco J Candido Dos Reis; Hélio H A Carrara; Cristhiam de Jesus H Martinez; Thais Gozzo; Daniela Bazan Palioto Journal: Support Care Cancer Date: 2021-06-08 Impact factor: 3.603
Authors: Pérola Michelle Vasconcelos Caribé; Cristina Cunha Villar; Guiseppe Alexandre Romito; Júlio Yoshio Takada; Ana Paula Pacanaro; Célia Maria Cassaro Strunz; Luiz Antonio Machado César; Antonio de Padua Mansur Journal: Ther Adv Chronic Dis Date: 2020-05-13 Impact factor: 5.091
Authors: Hye-Kyung Lee; Soo Jin Kim; Young Ho Kim; Youngkyung Ko; Suk Ji; Joo-Cheol Park Journal: BMC Oral Health Date: 2018-08-24 Impact factor: 2.757