Dao-Feng Zhang1, Xuebin Xu2, Qifa Song3, Yalong Bai1, Yi Zhang1, Minghui Song1, Chunlei Shi1, Xianming Shi1. 1. MOST-USDA Joint Research Center for Food Safety, School of Agriculture & Biology & State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, China. 2. Department of Microbiological Lab, Shanghai Municipal Center for Disease Control & Prevention, Shanghai, China. 3. Department of Microbiology, Ningbo Municipal Center for Disease Control & Prevention, Ningbo, Zhejiang, China.
Abstract
AIM: To investigate whether the Staphylococcus argenteus is present in Eastern China and to verify the utility of a new screening process. MATERIALS & METHODS: Phenotype observation, PCR assay targeting a hypothetical nonribosomal peptide synthetase (NRPS) gene, phylogenetic analysis of rpoB and multilocus sequence typing were used to screen and identify strains of S. argenteus from 839 presumptive S. aureus isolates. RESULTS: Eighty-nine (89/839, 10.6%) of the presumptive S. aureus isolates produced white colonies on tryptone soya agar plates. Of the white-colony isolates, six (6/89, 7%) were S. argenteus, 75 (75/89, 84%) were S. aureus and eight (8/89, 9%) were other bacteria. CONCLUSION: The PCR-based method targeting the NRPS gene can simultaneously identify and distinguish S. argenteus and S. aureus. All representative sequences of rpoB generated in this study were deposited in GenBank under accession numbers SJTU F20002, KT767581; SJTU F20269, KT767582; SJTU F20419, KT767583; SJTU F20420, KT767584; SJTU F20124, KT767585; SJTU F21164, KT767586; SJTU F21285, KT767587; SJTU F21224, KT767588; SJTU F21155, KT767589; SJTU F21294, KT767590; SJTU F20030, KT767591; SJTU F20044, KT767592; SJTU F20135, KT767593; SJTU F20123, KT767594; SJTU F21319, KT767595, respectively. All the new sequence types (STs) were submitted to a multilocus sequence typing database and the assigned ST numbers are ST3261 (151-469-20-101-145-150-131), ST3262 (12-3-1-1-4-4-410) and ST3267 (2-471-2-2-6-3-2).
AIM: To investigate whether the Staphylococcus argenteus is present in Eastern China and to verify the utility of a new screening process. MATERIALS & METHODS: Phenotype observation, PCR assay targeting a hypothetical nonribosomal peptide synthetase (NRPS) gene, phylogenetic analysis of rpoB and multilocus sequence typing were used to screen and identify strains of S. argenteus from 839 presumptive S. aureus isolates. RESULTS: Eighty-nine (89/839, 10.6%) of the presumptive S. aureus isolates produced white colonies on tryptone soya agar plates. Of the white-colony isolates, six (6/89, 7%) were S. argenteus, 75 (75/89, 84%) were S. aureus and eight (8/89, 9%) were other bacteria. CONCLUSION: The PCR-based method targeting the NRPS gene can simultaneously identify and distinguish S. argenteus and S. aureus. All representative sequences of rpoB generated in this study were deposited in GenBank under accession numbers SJTU F20002, KT767581; SJTU F20269, KT767582; SJTU F20419, KT767583; SJTU F20420, KT767584; SJTU F20124, KT767585; SJTU F21164, KT767586; SJTU F21285, KT767587; SJTU F21224, KT767588; SJTU F21155, KT767589; SJTU F21294, KT767590; SJTU F20030, KT767591; SJTU F20044, KT767592; SJTU F20135, KT767593; SJTU F20123, KT767594; SJTU F21319, KT767595, respectively. All the new sequence types (STs) were submitted to a multilocus sequence typing database and the assigned ST numbers are ST3261 (151-469-20-101-145-150-131), ST3262 (12-3-1-1-4-4-410) and ST3267 (2-471-2-2-6-3-2).
Authors: Bei Jiang; Bo You; Li Tan; Shengpeng Yu; Han Li; Guoqing Bai; Shu Li; Xiancai Rao; Zhao Xie; Xianming Shi; Yizhi Peng; Xiaomei Hu Journal: Front Microbiol Date: 2018-06-27 Impact factor: 5.640
Authors: Bruna F Rossi; Érika C R Bonsaglia; Ivana G Castilho; Stéfani T A Dantas; Hélio Langoni; José C F Pantoja; Ary Fernandes Júnior; Juliano L Gonçalves; Marcos V Santos; Rinaldo A Mota; Vera L M Rall Journal: BMC Vet Res Date: 2020-07-20 Impact factor: 2.741