AIM: There is increasing interest in the profiling and quantitation of methionine pathway metabolites for health management research. Currently, several analytical approaches are required to cover metabolites and co-factors. RESULTS: We report the development and the validation of a method for the simultaneous detection and quantitation of 13 metabolites in red blood cells. The method, validated in a cohort of healthy human volunteers, shows a high level of accuracy and reproducibility. CONCLUSION: This high-throughput protocol provides a robust coverage of central metabolites and co-factors in one single analysis and in a high-throughput fashion. In large-scale clinical settings, the use of such an approach will significantly advance the field of nutritional research in health and disease.
AIM: There is increasing interest in the profiling and quantitation of methionine pathway metabolites for health management research. Currently, several analytical approaches are required to cover metabolites and co-factors. RESULTS: We report the development and the validation of a method for the simultaneous detection and quantitation of 13 metabolites in red blood cells. The method, validated in a cohort of healthy human volunteers, shows a high level of accuracy and reproducibility. CONCLUSION: This high-throughput protocol provides a robust coverage of central metabolites and co-factors in one single analysis and in a high-throughput fashion. In large-scale clinical settings, the use of such an approach will significantly advance the field of nutritional research in health and disease.
Entities:
Keywords:
UPLC–MS/MS; high throughput; methionine pathway; one-carbon metabolism; red blood cells
Authors: Fábio V Ued; Mariana G Mathias; Roseli B D Toffano; Tamiris T Barros; Maria Olímpia R V Almada; Roberta G Salomão; Carolina A Coelho-Landell; Elaine Hillesheim; Joyce M Camarneiro; José Simon Camelo-Junior; Davi C Aragon; Sofia Moco; Martin Kussmann; Jim Kaput; Jacqueline P Monteiro Journal: Nutrients Date: 2019-12-02 Impact factor: 5.717
Authors: Amit Walia; Choongheon Lee; Jared Hartsock; Shawn S Goodman; Roland Dolle; Alec N Salt; Jeffery T Lichtenhan; Mark A Rutherford Journal: Front Synaptic Neurosci Date: 2021-07-05
Authors: Seu Ping Guiraud; Ivan Montoliu; Laeticia Da Silva; Loïc Dayon; Antonio Núñez Galindo; John Corthésy; Martin Kussmann; Francois-Pierre Martin Journal: Anal Bioanal Chem Date: 2016-10-18 Impact factor: 4.142
Authors: Roseli B D Toffano; Elaine Hillesheim; Mariana G Mathias; Carolina A Coelho-Landell; Roberta G Salomão; Maria O R V Almada; Joyce M Camarneiro; Tamiris T Barros; José S Camelo-Junior; Serge Rezzi; Laurence Goulet; Maria P Giner; Laeticia Da Silva; Francois-Pierre Martin; Ivan Montoliu; Sofia Moco; Sebastiano Collino; Jim Kaput; Jacqueline P Monteiro Journal: Nutrients Date: 2018-01-30 Impact factor: 5.717