Behrooz Soltani1, Narges Bodaghabadi2, Gita Mahpour2,3, Nasser Ghaemi4, Majid Sadeghizadeh2. 1. Department of Biotechnology, School of Chemistry, College of Science, University of Tehran, 14155-6455, Tehran, Iran. 2. Department of Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran, Iran. 3. Department of Biology, Azad University of Damghan, Damghan, Iran. 4. Department of Biotechnology, School of Chemistry, College of Science, University of Tehran, 14155-6455, Tehran, Iran. nasser.ghaemi@khayam.ut.ac.ir.
Abstract
OBJECTIVES: To investigated the potential of a novel dendrosomal nanoformulation of curcumin (DNC) in blocking radiation-induced changes in irradiated human umbilical vein endothelial cells (HUVECs), and their adhesion to human THP-1 monocytoid cells. RESULTS: Co60 gamma rays reduced viability, raised the expression of adhesion molecules, ICAM-1, VCAM-1 and E-selectin (mRNA and protein), augmented the adhesion of THP-1 cells to HUVECs, activated NF-κB binding, increased the release of pro-inflammatory cytokines (IL-6, IL-8 and MCP-1) and induced oxidative damage (reduced glutathione declined, while 8-OHdG and TBARS increased). 5 µM DNC significantly inhibited these radiation-induced changes, activated the Nrf-2 pathway, and effectively suppressed THP-1 adhesion to HUVECs, implicating p38 MAPK signaling. CONCLUSION: DNC treatment is a potential preventive method against inflammation and vascular damage from ionizing radiation.
OBJECTIVES: To investigated the potential of a novel dendrosomal nanoformulation of curcumin (DNC) in blocking radiation-induced changes in irradiated human umbilical vein endothelial cells (HUVECs), and their adhesion to humanTHP-1 monocytoid cells. RESULTS: Co60 gamma rays reduced viability, raised the expression of adhesion molecules, ICAM-1, VCAM-1 and E-selectin (mRNA and protein), augmented the adhesion of THP-1 cells to HUVECs, activated NF-κB binding, increased the release of pro-inflammatory cytokines (IL-6, IL-8 and MCP-1) and induced oxidative damage (reduced glutathione declined, while 8-OHdG and TBARS increased). 5 µM DNC significantly inhibited these radiation-induced changes, activated the Nrf-2 pathway, and effectively suppressed THP-1 adhesion to HUVECs, implicating p38 MAPK signaling. CONCLUSION:DNC treatment is a potential preventive method against inflammation and vascular damage from ionizing radiation.