| Literature DB >> 27557683 |
Ruyu Li1, Zhiguo Han1, Lili Hou1,2, Gurminder Kaur1,2, Qian Yin1,2, David W Ow3.
Abstract
Crop improvement is a never ending process. With a transgenesis approach, it is not inconceivable to envision a continuous addition of new transgenes to existing cultivars. Previously, we described a recombinase-directed gene stacking method in tobacco (Hou et al., Mol Plant 7:1756-1765, 2014). Being able to stack DNA to a previous location ensures that the number of genetic loci does not increase with each new round of transgene addition. Whereas the previous demonstration was conducted through polyethylene glycol to mediate uptake of DNA into tobacco protoplasts, we now describe protocols for using biolistic transformation to stack DNA in tobacco and rice.Entities:
Keywords: Gene stacking; Gene targeting; Recombinase; Site-specific recombination; Transformation; Transgene expression
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Year: 2016 PMID： 27557683 DOI： 10.1007/978-1-4939-4931-1_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745