André Luis Ribeiro Ribeiro1, Natacha Malu Miranda da Costa2, Adriane Sousa de Siqueira3, Walessa Brasil da Silva2, Maria Sueli da Silva Kataoka2, Ruy Gastaldoni Jaeger4, Sérgio de Melo Alves-Junior2, Andrew M Smith5, João de Jesus Viana Pinheiro2. 1. Department of Oral and Maxillofacial Surgery, School of Dentistry, University Center of Pará - CESUPA, Belém, Brazil; Department of Microbial Diseases, Eastman Dental Institute, University College London, London, England. Electronic address: Ribeiroalr@ig.com.br. 2. Cell Culture Laboratory, Faculty of Dentistry, Federal University of Pará, Belém, Brazil. 3. School of Dentistry, Positivo University, Curitiba, Brazil. 4. Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. 5. Department of Microbial Diseases, Eastman Dental Institute, University College London, London, England.
Abstract
OBJECTIVE: Keratocystic odontogenic tumor (KOT) is an odontogenic neoplasm that shows aggressive clinical behavior and local invasiveness. Invadopodia are actin-rich cellular protrusions exhibiting proteolytic pericellular activity, thereby inducing focal invasion in neoplastic cells and increasing neoplasms aggressiveness. Thus, this study aimed to evaluate immunoexpression of invadopodia-related proteins, cortactin, MT1-MMP, Tks4, and Tks5, in KOT. STUDY DESIGN: Immunohistochemistry of 16 cases of KOT, eight cases of calcifying cystic odontogenic tumor (CCOT), and eight samples of the oral mucosa (OM) was carried out to assess the expression of the above described invadopodia-related proteins in the basal and suprabasal layer. RESULTS: KOT samples showed higher and significant immunoexpression of cortactin, MT1-MMP, TKs4, and TKs5 compared with the CCOT and OM samples. Significant expression of all these proteins was observed in the basal layer compared with the suprabasal layer in KOT. CONCLUSIONS: Overexpression of cortactin, MT1-MMP, TKs4, and TKs5 was observed in KOT compared with samples of CCOT and OM. These proteins were also overexpressed in the basal over the suprabasal layer of KOT samples. Taken together, these results suggest the participation of invadopodia-related proteins on the pathogenesis of this lesion.
OBJECTIVE:Keratocystic odontogenic tumor (KOT) is an odontogenic neoplasm that shows aggressive clinical behavior and local invasiveness. Invadopodia are actin-rich cellular protrusions exhibiting proteolytic pericellular activity, thereby inducing focal invasion in neoplastic cells and increasing neoplasms aggressiveness. Thus, this study aimed to evaluate immunoexpression of invadopodia-related proteins, cortactin, MT1-MMP, Tks4, and Tks5, in KOT. STUDY DESIGN: Immunohistochemistry of 16 cases of KOT, eight cases of calcifying cystic odontogenic tumor (CCOT), and eight samples of the oral mucosa (OM) was carried out to assess the expression of the above described invadopodia-related proteins in the basal and suprabasal layer. RESULTS: KOT samples showed higher and significant immunoexpression of cortactin, MT1-MMP, TKs4, and TKs5 compared with the CCOT and OM samples. Significant expression of all these proteins was observed in the basal layer compared with the suprabasal layer in KOT. CONCLUSIONS: Overexpression of cortactin, MT1-MMP, TKs4, and TKs5 was observed in KOT compared with samples of CCOT and OM. These proteins were also overexpressed in the basal over the suprabasal layer of KOT samples. Taken together, these results suggest the participation of invadopodia-related proteins on the pathogenesis of this lesion.
Authors: Natacha M M da Costa; Adriane S de Siqueira; André L R Ribeiro; Maria S da Silva Kataoka; Ruy G Jaeger; Sérgio M de Alves-Júnior; Andrew M Smith; João de Jesus Viana Pinheiro Journal: Clin Oral Investig Date: 2017-02-25 Impact factor: 3.573
Authors: Natacha Malu Miranda da Costa; Caio Tadashi Saab Abe; Geovanni Pereira Mitre; Ricardo Alves Mesquita; Maria Sueli da Silva Kataoka; André Luis Ribeiro Ribeiro; Ruy Gastaldoni Jaeger; Sérgio de Melo Alves-Júnior; Andrew Mark Smith; João de Jesus Viana Pinheiro Journal: Cells Date: 2019-07-17 Impact factor: 6.600