Meryem Benzer1,2, Sebnem Tekin Neijmann3, Nazlı Dilay Gültekin4, Aslı Uluturk Tekin5. 1. Bakırköy Dr Sadi Konuk Training and Research Hospital, Division of Pediatric Nephrology, Istanbul, Turkey. mbenzer1@hotmail.com. 2. Bakırköy Dr Sadi Konuk Training and Research Hospital, Division of Pediatrics, İstanbul, Turkey. mbenzer1@hotmail.com. 3. Bakırköy Dr Sadi Konuk Training and Research Hospital, Division of Biochemistry, İstanbul, Turkey. 4. Bakırköy Dr Sadi Konuk Training and Research Hospital, Division of Pediatrics, İstanbul, Turkey. 5. European Commission, DG Joint Research Centre, Institute for Health and Consumer Protection, Ispra (Varese), Italy.
Abstract
PURPOSE: Liver-type fatty acid-binding protein is a small cytoplasmic protein which is expressed in the human renal proximal tubular epithelium and synthesized in response to renal tubular injury. The aim of the present study was to investigate the importance of urinary liver-type fatty acid-binding protein levels in children who diagnosed with vesicoureteral reflux. METHODS: Fifty-six patients with vesicoureteral reflux and 51 healthy controls were enrolled to the study. The cases were divided into three groups as follows: group A-the controls, group B-the patients who had renal parenchymal scarring and group C-the patients who had no scarring. Urinary liver-type fatty acid-binding protein was measured by enzyme-linked immunosorbent assay method. Creatinine was measured by modified Jaffe method, protein was measured by turbidimetric method, and urine density was determined by using the "falling drop" procedure. RESULTS: Urinary liver-type fatty acid-binding protein and urinary liver-type fatty acid-binding protein/creatinine levels were significantly higher in the whole patient group than in the controls (p = 0.016, 0.006). Significant differences were also determined by comparing the three groups (p = 0.015, 0.014), and those levels were found as significantly higher in group C. CONCLUSION: Urinary liver-type fatty acid-binding protein was considered to be helpful for the diagnosis of vesicoureteral reflux, and also it might contribute to understand the mechanisms causing scar tissue formation especially for the patients who had vesicoureteral reflux. Further clinical and experimental investigations are required to elucidate in detail the physiology of liver-type fatty acid-binding protein.
PURPOSE: Liver-type fatty acid-binding protein is a small cytoplasmic protein which is expressed in the human renal proximal tubular epithelium and synthesized in response to renal tubular injury. The aim of the present study was to investigate the importance of urinary liver-type fatty acid-binding protein levels in children who diagnosed with vesicoureteral reflux. METHODS: Fifty-six patients with vesicoureteral reflux and 51 healthy controls were enrolled to the study. The cases were divided into three groups as follows: group A-the controls, group B-the patients who had renal parenchymal scarring and group C-the patients who had no scarring. Urinary liver-type fatty acid-binding protein was measured by enzyme-linked immunosorbent assay method. Creatinine was measured by modified Jaffe method, protein was measured by turbidimetric method, and urine density was determined by using the "falling drop" procedure. RESULTS: Urinary liver-type fatty acid-binding protein and urinary liver-type fatty acid-binding protein/creatinine levels were significantly higher in the whole patient group than in the controls (p = 0.016, 0.006). Significant differences were also determined by comparing the three groups (p = 0.015, 0.014), and those levels were found as significantly higher in group C. CONCLUSION: Urinary liver-type fatty acid-binding protein was considered to be helpful for the diagnosis of vesicoureteral reflux, and also it might contribute to understand the mechanisms causing scar tissue formation especially for the patients who had vesicoureteral reflux. Further clinical and experimental investigations are required to elucidate in detail the physiology of liver-type fatty acid-binding protein.
Authors: Ivana Ivanišević; Amira Peco-Antić; Irena Vuličević; Dorđe Hercog; Vladimir Milovanović; Jelena Kotur-Stevuljević; Aleksandra Stefanović; Nikola Kocev Journal: Pediatr Nephrol Date: 2013-02-14 Impact factor: 3.714