| Literature DB >> 27548067 |
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Abstract
[This corrects the article DOI: 10.1371/journal.pone.0159603.].Entities:
Year: 2016 PMID: 27548067 PMCID: PMC4993501 DOI: 10.1371/journal.pone.0161420
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Predicted masses and primer pairs for the PfoTPT transfection constructs.
The tyrosine (Y) codon in synthetic PfoTPT and the alanine (A) codon in Y10A are in bold. The predicted mass given for TMD10 is for the entire TMD1+TMD10 fusion protein. The HindIII cloning sites are underlined. A HindIII site is naturally found within TMD1 and one was added to the TMD10 forward primer.
| Construct | Predicted mass | Forward primer | Reverse primer |
|---|---|---|---|
| Synthetic | 39.0 kDa | 5’CACCATGAAAGATAACGAAAAAAAAAACGAA | 5’AAAGATAGAGTATAGAAATGCTCCAAAGATGGC |
| Y10A | 38.9 kDa | 5’CACCATGAAAGATAACGAAAAAAAAAACGAA | 5’AAAGATAGAGTATAGAAATGCTCCAAAGATGGC |
| TMD1to9 | 39.5 kDa | 5’CACCTAAATAGAATGAAAGATAATGAAAAAA | 5’TATAATTATTGATGAAACAATGATAACA |
| TMD1to8 | 36.4 kDa | 5’CACCTAAATAGAATGAAAGATAATGAAAAAA | 5’AAGGCACATAAAAGCAACTTC |
| TMD1to6 | 27.0 kDa | 5’CACCTAAATAGAATGAAAGATAATGAAAAAA | 5’TGCATAAATAGATCTAATAGATGAACC |
| TMD1to5 | 24.0 kDa | 5’CACCTAAATAGAATGAAAGATAATGAAAAAA | 5’TTTCATAGATGCACAAACAACA |
| TMD1to2 | 14.2 kDa | 5’CACCTAAATAGAATGAAAGATAATGAAAAAA | 5’ACTTATCCAATATATAAATATAAATATCCAT |
| TMD1 | 10.8 kDa | 5’CACCTAAATAGAATGAAAGATAATGAAAAAA | 5’TA |
| TMD10 | 10.55 kDa | 5’CACC | 5’AAAGATTGAATACAAGAAAGCACCGAATATTG |