Literature DB >> 27548067

Correction: Targeting of a Transporter to the Outer Apicoplast Membrane in the Human Malaria Parasite Plasmodium falciparum.

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Abstract

[This corrects the article DOI: 10.1371/journal.pone.0159603.].

Entities:  

Year:  2016        PMID: 27548067      PMCID: PMC4993501          DOI: 10.1371/journal.pone.0161420

Source DB:  PubMed          Journal:  PLoS One        ISSN: 1932-6203            Impact factor:   3.240


There is an error in Table 1. The codons should be shown in bold. Please view the correct table here. The publisher apologizes for the error.

Predicted masses and primer pairs for the PfoTPT transfection constructs.

The tyrosine (Y) codon in synthetic PfoTPT and the alanine (A) codon in Y10A are in bold. The predicted mass given for TMD10 is for the entire TMD1+TMD10 fusion protein. The HindIII cloning sites are underlined. A HindIII site is naturally found within TMD1 and one was added to the TMD10 forward primer.
Table 1

Predicted masses and primer pairs for the PfoTPT transfection constructs.

The tyrosine (Y) codon in synthetic PfoTPT and the alanine (A) codon in Y10A are in bold. The predicted mass given for TMD10 is for the entire TMD1+TMD10 fusion protein. The HindIII cloning sites are underlined. A HindIII site is naturally found within TMD1 and one was added to the TMD10 forward primer.

ConstructPredicted massForward primerReverse primer
Synthetic PfoTPT39.0 kDa5’CACCATGAAAGATAACGAAAAAAAAAACGAATACGGTACGTT5’AAAGATAGAGTATAGAAATGCTCCAAAGATGGC
Y10A38.9 kDa5’CACCATGAAAGATAACGAAAAAAAAAACGAAGCTGGTACG5’AAAGATAGAGTATAGAAATGCTCCAAAGATGGC
TMD1to939.5 kDa5’CACCTAAATAGAATGAAAGATAATGAAAAAA5’TATAATTATTGATGAAACAATGATAACA
TMD1to836.4 kDa5’CACCTAAATAGAATGAAAGATAATGAAAAAA5’AAGGCACATAAAAGCAACTTC
TMD1to627.0 kDa5’CACCTAAATAGAATGAAAGATAATGAAAAAA5’TGCATAAATAGATCTAATAGATGAACC
TMD1to524.0 kDa5’CACCTAAATAGAATGAAAGATAATGAAAAAA5’TTTCATAGATGCACAAACAACA
TMD1to214.2 kDa5’CACCTAAATAGAATGAAAGATAATGAAAAAA5’ACTTATCCAATATATAAATATAAATATCCAT
TMD110.8 kDa5’CACCTAAATAGAATGAAAGATAATGAAAAAA5’TAAAGCTTTTTTATTATCTACATTATATAATAC
TMD1010.55 kDa5’CACCAAGCTTTATTTAAAACACAAATAACGTTACTTGGA5’AAAGATTGAATACAAGAAAGCACCGAATATTG
  1 in total

1.  Targeting of a Transporter to the Outer Apicoplast Membrane in the Human Malaria Parasite Plasmodium falciparum.

Authors:  Liting Lim; Claire P Sayers; Christopher D Goodman; Geoffrey I McFadden
Journal:  PLoS One       Date:  2016-07-21       Impact factor: 3.240

  1 in total

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