Data on biochemical indexes of HFD-fed mice treatment with metformin or resveratrol.

To investigate the changes of physiological and biochemical indexes, male mice were fed a regular diet or short time high fat diet (HFD) for 10 days with oral administration of saline, metformin, resveratrol, or injected intraperitoneally (ip) with digoxin respectively every day. Food intake and body weight were recorded simultaneously. Blood was collected after mice were sacrificed and then tested with commercial kits. The data manifested that metformin and resveratrol only ameliorate free fatty acids and glycerol in HFD-fed mice. Data interpretation of this part can be found in the research article "Metformin and resveratrol ameliorate muscle insulin resistance through preventing lipolysis and inflammation in hypoxic adipose tissue" (Zhao et al.,) [1].

Specifications Table

Value of the data

The data of short-term high-fat fed mice shows some differences between the data of regular chow diet fed mice.

The data demonstrates the different reason that caused tissue hypoxia [2].

The data could give a basis for further experiments on revealing the underlying mechanisms of relative adipocyte hypoxia [3].

Data

This article provides the data changes in food intake, body weight and serum indexes of the HFD-fed mice (Fig. 1).

Fig. 1

The effect of metformin and resveratrol treatment in HFD-fed mice.

Experimental design, materials and methods

Experimental design and animals

The animals design was as the same as described in section of “animals” in [1]. Food intake and body weight were recorded.

Measurement of blood lipids

CHOW-fed and HFD-fed mice were fasted for 8 h before collecting blood from the orbital sinus. FFA (Jiancheng, Nanjing, China), Glycerol (Jiancheng, Nanjing, China), TG (BHKT, Beijing, China) and TC (BHKT, Beijing, China) levels were tested according to the manufacturer׳s instructions.

Mice were fed with HFD for 10 days with oral administration of metformin (Met, 200 mg/kg), resveratrol (Res, 50 mg/kg), or intraperitoneal injection of digoxin (Dig, 1 mg/kg, as the control reagent). (A) Food intake of per mouse every day, Data were expressed as the mean±SD (n=9). (B) Body weights of CHOW-fed and HFD-fed mice every day (mean±SD, n=10). (C, D, E, F) Serum were collected after mice were killed. Free fatty acid (FFA), glycerol, total cholesterol (TC) and triglyceride (TG) were measured by commercial assay kits (mean±SD, n=5–6). *p<0.05 vs HFD feeding only treatment, #p<0.05 vs the indicated treatment.

Subject area	Biology
More specific subject area	High fat diet fed mice characterization
Type of data	Figure
How data was acquired	Food intake and body weight were recorded; biochemical indexes in serum were obtained with commercial kit and microplate reader
Data format	Analyzed
Experimental factors	Mice were fed a regular chow diet or HFD with oral administration of saline, metformin (200 mg/kg), resveratrol (50 mg/kg), or injected intraperitoneally (ip) with digoxin (1 mg/kg) respectively every day. Mice were fasted for 8 h before collecting blood from the orbital sinus.
Experimental features	Food intake and body weight were recorded. The measurement of the contents of free fatty acids (FFAs), glycerol, total cholesterol (TC) and triglyceride (TG) were performed using the commercial kits.
Data source location	Shanghai, China
Data accessibility	Data are presented in this article