| Literature DB >> 27543815 |
Alessia Galiero1, Filippo Fratini2, Cesare Cammà3, Marco Di Domenico3, Valentina Curini3, Irene Baronti2, Barbara Turchi2, Domenico Cerri2.
Abstract
Q fever is a zoonosis caused by Coxiella burnetii which infects humans as well as several animal species; sheep, goats and cattle are the primary animal reservoir. The main route of human exposure to Coxiella burnetii is inhalation of contaminated aerosols from excreta, especially birth products, while the role of unpasteurized dairy products in the transmission of Q fever to humans remains still controversial. The aim of this work was to evaluate the presence of Coxiella burnetii in unpasteurized cheese samples (n=84) by PCR and to genotype the circulating strains by Multispacer sequence typing (MST) analysis. Coxiella burnetii DNA was detected in 27/84 (32.14%) cheeses and positivity rate of handicraft cheeses reached 17.24%, while positivity rate of non-handicraft cheeses reached 65.38%. In addition, the MST profile of Coxiella burnetii detected in 5 cheese samples have shown the circulation of ST12 and ST32 genotypes in Tuscany.Entities:
Keywords: Cheese; Coxiella burnetii; Multispacer sequence typing; PCR; Small ruminants; Tuscany
Mesh：
Year: 2016 PMID： 27543815 DOI： 10.1016/j.ijfoodmicro.2016.08.008
Source DB: PubMed Journal: Int J Food Microbiol ISSN： 0168-1605 Impact factor: 5.277