Min-Kyung Yeo1, Min-Kyu Jung1, Su-Yel Lee2, Yong-Moon Lee1, Gang Min Hur3, Jin-Man Kim1. 1. Department of Pathology and Medical Science, Chungnam National University School of Medicine, Daejeon, Korea. 2. Biobank of Chungnam National University Hospital, Daejeon, Korea. 3. Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
Abstract
INTRODUCTION: The BRAF V600E mutation is the most common genetic event in papillary thyroid carcinoma (PTC). The BRAF V600E mutational status has a significant diagnostic and prognostic role in PTC since it can be detected in 32%-87% of PTC by various molecular methods. AIMS: A novel, fully automated real-time PCR-based Idylla test is assessed to detect the BRAF mutation in formalin-fixed paraffin-embedded (FFPE) thyroid samples. METHODS: 99 PTC and 11 nodular hyperplasia FFPE thyroid tissues are evaluated for the BRAF V600E mutation by the Idylla tests and compared with peptide nucleic acid-clamping PCR, real-time PCR and pyrosequencing. RESULTS: The sensitivity and specificity of the Idylla test to detect BRAF V600E are 98.8% and 100%, which is superior to real-time PCR and pyrosequencing. The concordance between Idylla and true positive is highest at 0.974. CONCLUSIONS: This study validates that the Idylla test is a sensitive and specific method to detect BRAF V600E in FFPE thyroid tissues. A simple, quick and easy to handle Idylla test is a useful and reliable molecular technique to evaluate BRAF mutations. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.
INTRODUCTION: The BRAFV600E mutation is the most common genetic event in papillary thyroid carcinoma (PTC). The BRAFV600E mutational status has a significant diagnostic and prognostic role in PTC since it can be detected in 32%-87% of PTC by various molecular methods. AIMS: A novel, fully automated real-time PCR-based Idylla test is assessed to detect the BRAF mutation in formalin-fixed paraffin-embedded (FFPE) thyroid samples. METHODS: 99 PTC and 11 nodular hyperplasia FFPE thyroid tissues are evaluated for the BRAFV600E mutation by the Idylla tests and compared with peptide nucleic acid-clamping PCR, real-time PCR and pyrosequencing. RESULTS: The sensitivity and specificity of the Idylla test to detect BRAFV600E are 98.8% and 100%, which is superior to real-time PCR and pyrosequencing. The concordance between Idylla and true positive is highest at 0.974. CONCLUSIONS: This study validates that the Idylla test is a sensitive and specific method to detect BRAFV600E in FFPE thyroid tissues. A simple, quick and easy to handle Idylla test is a useful and reliable molecular technique to evaluate BRAF mutations. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.
Authors: Louise Johnston; Michael Power; Philip Sloan; Anna Long; Angela Silmon; Ben Chaffey; Andrea Jane Lisgo; Liam Little; Ellen Vercauteren; Torben Steiniche; Tine Meyer; John Simpson Journal: J Clin Pathol Date: 2017-09-12 Impact factor: 3.411
Authors: Mercedes Delgado-García; Birgit Weynand; Lourdes Gómez-Izquierdo; María José Hernández; Ángela María Blanco; Mar Varela; Xavier Matias-Guiu; Ernest Nadal; Bélgica Márquez-Lobo; Ana Alarcão; Enrique de Álava; Michele Biscuola Journal: BMC Cancer Date: 2020-04-03 Impact factor: 4.430