Ádám Solti1, Krisztina Kovács2, Brigitta Müller3, Saúl Vázquez4,5, Éva Hamar3, Hong Diep Pham3, Brigitta Tóth6, Javier Abadía4, Ferenc Fodor3. 1. Department of Plant Physiology and Molecular Plant Biology, Institute of Biology, Faculty of Sciences, Eötvös Loránd University, Pázmány P. Sétány 1/C, Budapest, 1117, Hungary. adam.solti@ttk.elte.hu. 2. Laboratory of Nuclear Chemistry, Department of Analytical Chemistry, Institute of Chemistry, Faculty of Sciences, Eötvös Loránd University, Pázmány P. Sétány 1/A, Budapest, 1117, Hungary. 3. Department of Plant Physiology and Molecular Plant Biology, Institute of Biology, Faculty of Sciences, Eötvös Loránd University, Pázmány P. Sétány 1/C, Budapest, 1117, Hungary. 4. Department of Plant Nutrition, Aula Dei Experimental Station, Spanish Council for Scientific Research (CSIC), P.O. Box 13034, 50080, Saragossa, Spain. 5. Faculty of Science, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire, LE12 5RD, UK. 6. Department of Agricultural Botany, Crop Physiology and Biotechnology, Institute of Crop Sciences, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Böszörményi út 138, Debrecen, 4032, Hungary.
Abstract
MAIN CONCLUSION: Based on the effects of inorganic salts on chloroplast Fe uptake, the presence of a voltage-dependent step is proposed to play a role in Fe uptake through the outer envelope. Although iron (Fe) plays a crucial role in chloroplast physiology, only few pieces of information are available on the mechanisms of chloroplast Fe acquisition. Here, the effect of inorganic salts on the Fe uptake of intact chloroplasts was tested, assessing Fe and transition metal uptake using bathophenantroline-based spectrophotometric detection and plasma emission-coupled mass spectrometry, respectively. The microenvironment of Fe was studied by Mössbauer spectroscopy. Transition metal cations (Cd2+, Zn2+, and Mn2+) enhanced, whereas oxoanions (NO3-, SO42-, and BO33-) reduced the chloroplast Fe uptake. The effect was insensitive to diuron (DCMU), an inhibitor of chloroplast inner envelope-associated Fe uptake. The inorganic salts affected neither Fe forms in the uptake assay buffer nor those incorporated into the chloroplasts. The significantly lower Zn and Mn uptake compared to that of Fe indicates that different mechanisms/transporters are involved in their acquisition. The enhancing effect of transition metals on chloroplast Fe uptake is likely related to outer envelope-associated processes, since divalent metal cations are known to inhibit Fe2+ transport across the inner envelope. Thus, a voltage-dependent step is proposed to play a role in Fe uptake through the chloroplast outer envelope on the basis of the contrasting effects of transition metal cations and oxoaninons.
MAIN CONCLUSION: Based on the effects of inorganic salts on chloroplast Fe uptake, the presence of a voltage-dependent step is proposed to play a role in Fe uptake through the outer envelope. Although iron (Fe) plays a crucial role in chloroplast physiology, only few pieces of information are available on the mechanisms of chloroplast Fe acquisition. Here, the effect of inorganic salts on the Fe uptake of intact chloroplasts was tested, assessing Fe and transition metal uptake using bathophenantroline-based spectrophotometric detection and plasma emission-coupled mass spectrometry, respectively. The microenvironment of Fe was studied by Mössbauer spectroscopy. Transition metal cations (Cd2+, Zn2+, and Mn2+) enhanced, whereas oxoanions (NO3-, SO42-, and BO33-) reduced the chloroplast Fe uptake. The effect was insensitive to diuron (DCMU), an inhibitor of chloroplast inner envelope-associated Fe uptake. The inorganic salts affected neither Fe forms in the uptake assay buffer nor those incorporated into the chloroplasts. The significantly lower Zn and Mn uptake compared to that of Fe indicates that different mechanisms/transporters are involved in their acquisition. The enhancing effect of transition metals on chloroplast Fe uptake is likely related to outer envelope-associated processes, since divalent metal cations are known to inhibit Fe2+ transport across the inner envelope. Thus, a voltage-dependent step is proposed to play a role in Fe uptake through the chloroplast outer envelope on the basis of the contrasting effects of transition metal cations and oxoaninons.
Entities:
Keywords:
Chloroplast; Envelope membrane; Iron metabolism; Mössbauer spectrosopy; Voltage-dependent transport
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