Literature DB >> 27540601

Fluorescence detection of single-nucleotide differences using aptamer-forming binary DNA probes.

Saori Kamoto1, Masumi Hyuga1, Teru Kato1.   

Abstract

We report a simple method for fluorescence detection of single-nucleotide alterations in a long target DNA, which is based on the formation of a three-way-junction-structured cholic-acid-binding DNA aptamer by the hybridization of the target with binary DNA probes. The new method was successfully exploited for SNP genotyping of human CYP2C19 gene.

Entities:  

Mesh:

Substances:

Year:  2016        PMID: 27540601     DOI: 10.1039/c6an00912c

Source DB:  PubMed          Journal:  Analyst        ISSN: 0003-2654            Impact factor:   4.616


  1 in total

1.  The linkers in fluorene-labeled 2'-deoxyuridines affect fluorescence discriminating phenomena upon duplex formation.

Authors:  So Young Lee; Seung Woo Hong; Hyeonuk Yeo; Gil Tae Hwang
Journal:  RSC Adv       Date:  2020-05-19       Impact factor: 3.361

  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.