Ahmadreza Nobakhti-Afshar1, Alireza Najafpour1, Rahim Mohammadi2, Leila Zarei3. 1. Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran. 2. Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. 3. Solid Tumor Research Center, Urmia University of Medical Sciences, Urmia, Iran.
Abstract
OBJECTIVE: To assess the neuroprotective effects of local administration of 17- beta- estradiol on nerve regeneration. METHODS: Sixty female Wistar rats were overiectomized and divided into four experimental groups (n = 15), randomly: In autograft group a segment of sciatic nerve was transected and re-implanted reversely. In sham-surgery group sciatic nerve was exposed and manipulated. In transected group left sciatic nerve was transected and stumps were fixed in adjacent muscle. In treatment group defect was bridged using a silicon conduit filled with 10 µL (0.1 mg/mL) 17- beta- estradiol. Each group was subdivided into four subgroups of five animals each and nerve fibers were studied in a 12-week period. RESULTS: Behavioral, functional, biomechanical, electrophysiological and gastrocnemius muscle mass findings and morphometric indices confirmed faster recovery of regenerated axons in treatment group than in other groups (p<0.05). Immunohistochemical reactions to S-100 in treatment group were more positive than that in other groups. CONCLUSION: Local administration of 17-beta-estradiol improved functional recovery and morphometric indices of sciatic nerve. It could have clinical implications for the surgical management of patients after facial nerve transection.
OBJECTIVE: To assess the neuroprotective effects of local administration of 17- beta- estradiol on nerve regeneration. METHODS: Sixty female Wistar rats were overiectomized and divided into four experimental groups (n = 15), randomly: In autograft group a segment of sciatic nerve was transected and re-implanted reversely. In sham-surgery group sciatic nerve was exposed and manipulated. In transected group left sciatic nerve was transected and stumps were fixed in adjacent muscle. In treatment group defect was bridged using a silicon conduit filled with 10 µL (0.1 mg/mL) 17- beta- estradiol. Each group was subdivided into four subgroups of five animals each and nerve fibers were studied in a 12-week period. RESULTS: Behavioral, functional, biomechanical, electrophysiological and gastrocnemius muscle mass findings and morphometric indices confirmed faster recovery of regenerated axons in treatment group than in other groups (p<0.05). Immunohistochemical reactions to S-100 in treatment group were more positive than that in other groups. CONCLUSION: Local administration of 17-beta-estradiol improved functional recovery and morphometric indices of sciatic nerve. It could have clinical implications for the surgical management of patients after facial nerve transection.
Entities:
Keywords:
17-beta-estradiol; Local administration; Nerve regeneration; Rat; Sciatic nerve
Authors: Lucila G Caceres; Soledad L Uran; María A Zorrilla Zubilete; Juan I Romero; Francisco Capani; Laura R Guelman Journal: J Neurochem Date: 2011-06-24 Impact factor: 5.372
Authors: Rustem R Islamov; Wesley A Hendricks; Robert J Jones; Gregory J Lyall; Nicole S Spanier; Alexander K Murashov Journal: Brain Res Date: 2002-07-12 Impact factor: 3.252
Authors: D Michele Basso; Lesley C Fisher; Aileen J Anderson; Lyn B Jakeman; Dana M McTigue; Phillip G Popovich Journal: J Neurotrauma Date: 2006-05 Impact factor: 5.269
Authors: Max Modrak; M A Hassan Talukder; Khatuna Gurgenashvili; Mark Noble; John C Elfar Journal: J Neurosci Res Date: 2019-10-13 Impact factor: 4.164
Authors: Fereshteh Khomejani Farahani; Hamidreza Fattahian; Ahmad Asghari; Pejman Mortazavi Journal: Vet Res Forum Date: 2022-06-15 Impact factor: 0.950