| Literature DB >> 27539462 |
Yang-Yi Fan1, Laurie A Davidson1,2, Robert S Chapkin3,4.
Abstract
Colonic organoids, three-dimensional colonic crypts grown in vitro that show realistic microanatomy, have many potential applications for studying physiology, developmental biology, and pathophysiology of intestinal diseases including inflammatory bowel disease and colorectal cancer. Here, we describe detailed protocols for mouse colonic crypt isolation, organoid culture, and downstream applications. Specific culture strategies including growth factor enriched Matrigel and Wnt and R-spondin conditioned media serve as key factors for enhancing the growth and cost efficiency of colonic organoid cultures.Entities:
Keywords: In vitro primary culture; Niche-independent crypt expansion; Stem cells
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Year: 2019 PMID： 27539462 PMCID： PMC5316509 DOI： 10.1007/7651_2016_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745