INTRODUCTION: Skeletal muscle microRNAs (miRNAs) are potential candidate biomarkers for amyotrophic lateral sclerosis (ALS) that deserve further investigation. OBJECTIVES: To identify miRNAs abnormally expressed in the skeletal muscle and plasma of patients with ALS, and to correlate them with parameters of disease progression. METHODS: Expression profile of miRNAs in muscle was evaluated using an array platform. Subsequently we assessed the plasmatic expression of candidate miRNAs in a set of 39 patients/39 controls. We employed generalized estimating equations to investigate correlations with clinical data. RESULTS: We identified 11 miRNAs differentially expressed in the muscle of ALS patients; of these, miR424, miR-214 and miR-206 were validated by qPCR in muscle samples. In plasma, we found only miR-424 and miR 206 to be overexpressed. Baseline expression of miR-424 and 206 correlated with clinical deterioration over time. CONCLUSION: MiR-424 and miR-206 are potential prognostic markers for ALS.
INTRODUCTION: Skeletal muscle microRNAs (miRNAs) are potential candidate biomarkers for amyotrophic lateral sclerosis (ALS) that deserve further investigation. OBJECTIVES: To identify miRNAs abnormally expressed in the skeletal muscle and plasma of patients with ALS, and to correlate them with parameters of disease progression. METHODS: Expression profile of miRNAs in muscle was evaluated using an array platform. Subsequently we assessed the plasmatic expression of candidate miRNAs in a set of 39 patients/39 controls. We employed generalized estimating equations to investigate correlations with clinical data. RESULTS: We identified 11 miRNAs differentially expressed in the muscle of ALSpatients; of these, miR424, miR-214 and miR-206 were validated by qPCR in muscle samples. In plasma, we found only miR-424 and miR 206 to be overexpressed. Baseline expression of miR-424 and 206 correlated with clinical deterioration over time. CONCLUSION:MiR-424 and miR-206 are potential prognostic markers for ALS.
Authors: Iddo Magen; Nancy Sarah Yacovzada; Eran Yanowski; Anna Coenen-Stass; Julian Grosskreutz; Ching-Hua Lu; Linda Greensmith; Andrea Malaspina; Pietro Fratta; Eran Hornstein Journal: Nat Neurosci Date: 2021-10-28 Impact factor: 24.884
Authors: Zachary C E Hawley; Danae Campos-Melo; Cristian A Droppelmann; Michael J Strong Journal: Front Mol Neurosci Date: 2017-05-04 Impact factor: 5.639
Authors: Greig Joilin; Elizabeth Gray; Alexander G Thompson; Yoana Bobeva; Kevin Talbot; Jochen Weishaupt; Albert Ludolph; Andrea Malaspina; P Nigel Leigh; Sarah F Newbury; Martin R Turner; Majid Hafezparast Journal: Brain Commun Date: 2020-06-17