Yannan Liu1,2, Zhiqiang Mi3, Wenkai Niu2, Xiaoping An3, Xin Yuan2, Huiying Liu2, Yong Wang2, Yuzhong Feng2, Yong Huang3, Xianglilan Zhang3, Zhiyi Zhang3, Hang Fan3, Fan Peng4, Puyuan Li2, Yigang Tong3, Changqing Bai2. 1. Department of Graduate, Hebei North University, Zhangjiakou, Hebei 075000, China. 2. Department of Respiratory & Critical Care Diseases, 307th Hospital of PLA, Beijing 100071, China. 3. Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China. 4. Department of Respiratory Medicine, the First Hospital of Changsha, Changsha, Hunan 410000, China.
Abstract
AIM: The ability of Acinetobacter baumannii to form biofilms and develop antibiotic resistance makes it difficult to control infections caused by this bacterium. In this study, we explored the potential of a lytic bacteriophage to disrupt A. baumannii biofilms. MATERIALS & METHODS: The potential of the lytic bacteriophage to disrupt A. baumannii biofilms was assessed by performing electron microscopy, live/dead bacterial staining, crystal violet staining and by determining adenosine triphosphate release. RESULTS: The bacteriophage inhibited the formation of and disrupted preformed A. baumannii biofilms. Results of disinfection assay showed that the lytic bacteriophage lysed A. baumannii cells suspended in blood or grown on metal surfaces. CONCLUSION: These results suggest the potential of the lytic bacteriophage to disrupt A. baumannii biofilms.
AIM: The ability of Acinetobacter baumannii to form biofilms and develop antibiotic resistance makes it difficult to control infections caused by this bacterium. In this study, we explored the potential of a lytic bacteriophage to disrupt A. baumannii biofilms. MATERIALS & METHODS: The potential of the lytic bacteriophage to disrupt A. baumannii biofilms was assessed by performing electron microscopy, live/dead bacterial staining, crystal violet staining and by determining adenosine triphosphate release. RESULTS: The bacteriophage inhibited the formation of and disrupted preformed A. baumannii biofilms. Results of disinfection assay showed that the lytic bacteriophage lysed A. baumannii cells suspended in blood or grown on metal surfaces. CONCLUSION: These results suggest the potential of the lytic bacteriophage to disrupt A. baumannii biofilms.
Authors: L Blasco; I Bleriot; M González de Aledo; L Fernández-García; O Pacios; H Oliveira; M López; C Ortiz-Cartagena; F Fernández-Cuenca; Á Pascual; L Martínez-Martínez; J Pachón; J Azeredo; M Tomás Journal: Antimicrob Agents Chemother Date: 2022-01-18 Impact factor: 5.938