Jalil Karnoosh-Yamchi1,2,3, Mohammad Rahmati-Yamchi1,2,3, Abolfazl Akbarzadeh4, Soodabeh Davaran4, Ali Reza Ostad Rahimi1, Khalil Garnoosh5, Zahra Bahmani6, Mohammad Ashoori2, Majid Mobasseri7. 1. a Nutrition Research Center , Tabriz University of Medical Sciences , Tabriz , Iran. 2. b Department of Clinical Biochemistry, Faculty of Medicine , Tabriz University of Medical Sciences , Tabriz , Iran. 3. c Aras International Branch of Tabriz University of Medical Sciences , Tabriz , Iran. 4. d Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran. 5. e Department of Neurology, Faculty of Medicine , Neuroscience Research Center (NSRC), Tabriz University of Medical Sciences , Tabriz , Iran. 6. f Plant Science Department , University of Tabriz , Tabriz , Iran. 7. g Bone Health Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.
Abstract
BACKGROUND: There are different methods for insulin administration in diabetic patient. Nano-hydrogel is one of the most talented drug carrier for its sensitivity to environmental stimulus. METHODS AND RESULTS: NIPAAm-MAA-HEM copolymers were synthesized by radical chain reaction. The copolymers were characterized with Scanning electron microscopy (SEM) and Transient electron microscopy (TEM). Copolymers were loaded with regular insulin by modified double emulsion method. Diabetic rats are used for feeding insulin-loaded nanohydrogel. Analysis of the results from the measurement of the amount of blood insulin from the rats blood that received insulin in nanohydrogel loaded form compared with rats that received pure insulin is significantly high, which confirm that insulin has been able to pass from the stomach acid barrier by nanohydrogel and is absorbed from the intestine. Blood sugar levels from tested rats indicate that with increasing amount of insulin, blood sugar levels fall down. CONCLUSION: Our study confirms that insulin has been able to pass from the stomach acid barrier by nanohydrogel and be absorbed from the intestine.
BACKGROUND: There are different methods for insulin administration in diabeticpatient. Nano-hydrogel is one of the most talented drug carrier for its sensitivity to environmental stimulus. METHODS AND RESULTS:NIPAAm-MAA-HEM copolymers were synthesized by radical chain reaction. The copolymers were characterized with Scanning electron microscopy (SEM) and Transient electron microscopy (TEM). Copolymers were loaded with regular insulin by modified double emulsion method. Diabeticrats are used for feeding insulin-loaded nanohydrogel. Analysis of the results from the measurement of the amount of blood insulin from the rats blood that received insulin in nanohydrogel loaded form compared with rats that received pure insulin is significantly high, which confirm that insulin has been able to pass from the stomach acid barrier by nanohydrogel and is absorbed from the intestine. Blood sugar levels from tested rats indicate that with increasing amount of insulin, blood sugar levels fall down. CONCLUSION: Our study confirms that insulin has been able to pass from the stomach acid barrier by nanohydrogel and be absorbed from the intestine.
Entities:
Keywords:
PH-sensitive nanohydrogels; diabetes mellitus; drug delivery; insulin
Authors: Mohd Mujeeb; Abdul Ahad; Mohd Aqil; Waseem Ahmad Siddiqui; Abul Kalam Najmi; Mymoona Akhtar; Apeksha Shrivastava; Abdul Qadir; Thasleem Moolakkadath Journal: Drug Deliv Transl Res Date: 2021-05-19 Impact factor: 4.617