| Literature DB >> 2753164 |
Y Vandenberghe1, F Morel, A Foriers, B Ketterer, A Vercruysse, A Guillouzo, V Rogiers.
Abstract
Cultured adult rat hepatocytes were treated daily with 3.2 mM phenobarbital (PB) in order to study its effect on the expression of cytosolic glutathione S-transferase isoenzymes. Glutathione S-transferase (GST) activities, using 1-chloro-2,4-dinitrobenzene and 1,2-dichloro-4-nitrobenzene as substrates, were increased when PB was present in the culture medium. After purification and separation of GST on glutathione Sepharose 6 B and reversed-phase HPLC, respectively, it was observed in vitro that PB caused an increase in the relative amounts of subunits 1, 3 and 7 compared to subunits 2 and 4. Using Northern blot technique, elevated levels of GST subunit 1/2 and 7 mRNA were measured, after addition of PB to the cultures.Entities:
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Year: 1989 PMID： 2753164 DOI： 10.1016/0014-5793(89)81428-0
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124