| Literature DB >> 27530632 |
Guang-Jie Song1,2, Su-Yun Bai3,4, Jing Luo1, Xiao-Qun Cao5, Bao-Xiang Zhao6.
Abstract
A new ratiometric fluorescent probe based on Förster resonance energy transfer (FRET) for sensing lysosomal pH has been developed. The probe (RMPM) was composed of imidazo[1,5-α]pyridine quaternary ammonium salt fluorophore as the FRET donor and the rhodamine moiety as the FRET acceptor. It's the first time to report that imidazo[1,5-α]pyridine quaternary ammonium salt acts as the FRET donor. The ratio of fluorescence intensity of the probe at two wavelengths (I424/I581) changed significantly and responded linearly toward minor pH changes in the range of 5.4-6.6. It should be noted that it's rare to report that a ratiometric pH probe could detect so weak acidic pH with pKa = 6.31. In addition, probe RMPM exhibited excellent water-solubility, fast-response, all-right selectivity and brilliant reversibility. Moreover, RMPM has been successfully applied to sensing lysosomal pH in HeLa cells and has low cytotoxicity.Entities:
Keywords: Image; Imidazo[1,5-α]pyridine quaternary ammonium salt; Lysosomal pH; Ratiometric fluorescent probe; Water-solubility
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Year: 2016 PMID： 27530632 DOI： 10.1007/s10895-016-1902-4
Source DB: PubMed Journal: J Fluoresc ISSN： 1053-0509 Impact factor: 2.217