C N Portela1, A Schriefer2, S R L Albuquerque1,3, R T Perdomo4, A F A Parente5, S S Weber6. 1. Pós-Graduação em Ciências Aplicadas à Hematologia, Universidade do Estado do Amazonas e Fundação de Hematologia e Hemoterapia do Amazonas, Manaus, Brazil. 2. Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia; Departamento de Ciências da Biointeração, Instituto de Ciências da Saúde, Instituto de Ciência e Tecnologia em Doenças Tropicais, Salvador, Brazil. 3. Laboratório de Imunohematologia Molecular, Fundação de Hematologia e Hemoterapia do Amazonas, Manaus, Brazil. 4. Centro de Ciências Biológicas e da Saúde, Universidade Federal do Mato Grosso do Sul, Campo Grande, Brazil. 5. Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Manaus, Brazil. 6. Instituto de Ciências Exatas e Tecnologia, Universidade Federal do Amazonas, Itacoatiara, Brazil.
Abstract
BACKGROUND: Human platelet antigens (HPAs) are alloantigens derived from polymorphisms in platelet-surface glycoproteins. The occurrence of alloantibodies against HPAs can lead to platelet destruction and subsequent thrombocytopenia. Brazilians have a high rate of racial admixture, and the knowledge of HPA polymorphisms in particular donors from north Brazil, who have a large Amerindian influence, is a relevant strategy to prevent alloimmunisation. OBJECTIVE: Our aim was investigate the HPA allele's frequencies in the Amazonas blood donors. METHODS: We performed HPA genotyping among 200 Amazonas blood donors by microarray for 11 HPA biallelic systems, including six of the most clinically significant systems (HPA-1 to -5 and -15) and five others (HPA-6 to -9 and -11) that have been also associated with alloimmunisation, amounting to 22 HPA alleles. RESULTS: The obtained allele frequencies were compared with data of 38 populations worldwide to determine the hierarchical relationship and estimated the probability of mismatch platelets. The allele frequencies were 0·862 for HPA-1a, 0·137 for HPA-1b, 0·852 for HPA-2a, 0·147 for HPA-2b, 0·665 for HPA-3a, 0·335 for HPA-3b, 0·995 for HPA-4a, 0·005 for HPA-4b, 0·892 for HPA-5a, 0·107 for HPA-5b, 0·997 for HPA-9a, 0·005 for HPA-9b, 0·502 for HPA-15a and 0·497 for HPA-15b. The incompatibility risks are higher for HPA-15 and HPA-3, followed by HPA-1, -2 and -5. CONCLUSION: We found differences among populations worldwide, and it is interesting to note the indigenous and European influences in this region, reinforcing the heterogeneity in the ancestry of Brazilians. The results will be helpful in providing information for platelet transfusion to avoid alloimmunisation.
BACKGROUND:Human platelet antigens (HPAs) are alloantigens derived from polymorphisms in platelet-surface glycoproteins. The occurrence of alloantibodies against HPAs can lead to platelet destruction and subsequent thrombocytopenia. Brazilians have a high rate of racial admixture, and the knowledge of HPA polymorphisms in particular donors from north Brazil, who have a large Amerindian influence, is a relevant strategy to prevent alloimmunisation. OBJECTIVE: Our aim was investigate the HPA allele's frequencies in the Amazonas blood donors. METHODS: We performed HPA genotyping among 200 Amazonas blood donors by microarray for 11 HPA biallelic systems, including six of the most clinically significant systems (HPA-1 to -5 and -15) and five others (HPA-6 to -9 and -11) that have been also associated with alloimmunisation, amounting to 22 HPA alleles. RESULTS: The obtained allele frequencies were compared with data of 38 populations worldwide to determine the hierarchical relationship and estimated the probability of mismatch platelets. The allele frequencies were 0·862 for HPA-1a, 0·137 for HPA-1b, 0·852 for HPA-2a, 0·147 for HPA-2b, 0·665 for HPA-3a, 0·335 for HPA-3b, 0·995 for HPA-4a, 0·005 for HPA-4b, 0·892 for HPA-5a, 0·107 for HPA-5b, 0·997 for HPA-9a, 0·005 for HPA-9b, 0·502 for HPA-15a and 0·497 for HPA-15b. The incompatibility risks are higher for HPA-15 and HPA-3, followed by HPA-1, -2 and -5. CONCLUSION: We found differences among populations worldwide, and it is interesting to note the indigenous and European influences in this region, reinforcing the heterogeneity in the ancestry of Brazilians. The results will be helpful in providing information for platelet transfusion to avoid alloimmunisation.
Authors: Maria Clara Fernandes Silva-Malta; Lucas Gabriel Tavares de Oliveira; Luísa Ferreira Barreiros; Dilson Rocha do Amaral; Marina Lobato Martins Journal: Transfus Med Hemother Date: 2018-05-11 Impact factor: 3.747
Authors: Julia Cavalcante do Carmo; Prissyla de Souza Klippel; Sabrine da Costa Cordeiro; Ângela Maria Dos Santos Fernandes; Raquel Medeiros Pinto; Simone Schneider Weber; Cleiton Fantin Journal: Rev Bras Hematol Hemoter Date: 2017-02-22