Wee Ling Koh1, Phoebe Huijun Tham2, Hanry Yu3,4,5,6, Hwa Liang Leo1,4, James Chen Yong Kah1,4. 1. Department of Biomedical Engineering, National University of Singapore, Singapore. 2. Interdisciplinary Graduate School, Nanyang Technological University, Singapore. 3. Department of Physiology, National University of Singapore, Singapore. 4. NUS Graduate School for Integrative Sciences & Engineering, National University of Singapore, Singapore. 5. Institute of Bioengineering & Nanotechnology, Agency for Science, Technology & Research, Singapore. 6. Mechanobiology Institute, National University of Singapore, Singapore.
Abstract
AIM: We examined the impact of aggregation and protein corona formation of gold nanoparticles (AuNPs) on the cytotoxicity, uptake and metabolism, specifically urea and albumin synthesis, of primary rat hepatocytes. MATERIALS & METHODS: The AuNPs were synthesized via citrate reduction and the human serum protein corona was preformed on the AuNPs. Primary hepatocytes were isolated from male Wistar rats via two-step in situ collagenase perfusion method, and were dosed with both citrate-capped (AuNP-Cit) and protein corona coated AuNPs (AuNP-Cor). RESULTS: The AuNP-Cor showed higher cell uptake and reduced cell viability compared with aggregated AuNP-Cit. Urea and albumin secretions showed AuNP dose dependency. Both AuNP-Cit and AuNP-Cor exerted only an acute effect on the albumin synthesis of hepatocytes with no chronic impact.
AIM: We examined the impact of aggregation and protein corona formation of gold nanoparticles (AuNPs) on the cytotoxicity, uptake and metabolism, specifically urea and albumin synthesis, of primary rat hepatocytes. MATERIALS & METHODS: The AuNPs were synthesized via citrate reduction and the human serum protein corona was preformed on the AuNPs. Primary hepatocytes were isolated from male Wistar rats via two-step in situ collagenase perfusion method, and were dosed with both citrate-capped (AuNP-Cit) and protein corona coated AuNPs (AuNP-Cor). RESULTS: The AuNP-Cor showed higher cell uptake and reduced cell viability compared with aggregated AuNP-Cit. Urea and albumin secretions showed AuNP dose dependency. Both AuNP-Cit and AuNP-Cor exerted only an acute effect on the albumin synthesis of hepatocytes with no chronic impact.
Entities:
Keywords:
gold nanoparticles; hepatotoxicity; liver toxicology; primary rat hepatocytes