J Deng1, J Yu. 1. Intensive Care Unit, the Second Hospital of Dalian Medical University, Dalian 116027, China.
Abstract
OBJECTIVE: To investigate the effects of rosiglitazone on the structure of the kidney of rats in sepsis with acute kidney injury. METHODS: Sepsis models were established using male Sprague-Dawley rats by cecal ligation puncture (CLP). 96 healthy rats were randomly divided into 4 groups: cecal ligation puncture and rosiglitazone group (group CLP+ ROSI), cecal ligation puncture group (group CLP), Sham and rosiglitazone group (group Sham+ ROSI), sham group (group Sham). Six rats were sacrificed at 0, 6, 12, 24 h after CLP respectively in each group. The general status of rats was observed and H&E staining was applied to detect renal pathological histology changes. Myeloperoxidase(MPO) was measured in kidney tissues. Expression of the ligand of peroxisome proliferators-activated receptor γ (PPARγ), and B-cell lymphoma-2(Bcl-2) were also determined by Western blot analysis. RESULTS: The increased MPO activity of group CLP+ ROSI was significantly lower than that of group CLP at 12 h and 24 h[(4.07±0.16)vs(4.62±0.08); P12=0.01, P24=0.006]. The MPO activity of group CLP was significantly higher than that of group Sham at 6, 12 and 24 h[(4.62±0.08)vs(1.30±0.06); P6=0.00, P12=0.00, P24=0.00]. The MPO activity of group CLP+ ROSI was significantly higher than that of group Sham+ ROSI at 6, 12 and 24 h[(4.07±0.16)vs(1.27±0.06); P6=0.00, P12=0.00, P24=0.00]. Compared with group Sham and group Sham+ ROSI, in rats of group CLP and group CLP+ ROSI the symptoms of sepsis and organ dysfunction were observed. The symptoms of sepsis and organ dysfunction of group CLP+ ROSI were significantly reduced than that of group CLP. Expression of PPARγ and Bcl-2 were determined by Western blot analysis, the expression of PPARγ and Bcl-2 of group CLP+ ROSI was significantly lower than that of group CLP. CONCLUSION: Rosiglitazone, as the ligand of PPARγ, reduced the inflammatory response in sepsis, decreased the apoptosis of kidney cell, reduced kidney injury, and protected acute kidney injury in rats of sepsis.
OBJECTIVE: To investigate the effects of rosiglitazone on the structure of the kidney of rats in sepsis with acute kidney injury. METHODS:Sepsis models were established using male Sprague-Dawley rats by cecal ligation puncture (CLP). 96 healthy rats were randomly divided into 4 groups: cecal ligation puncture and rosiglitazone group (group CLP+ ROSI), cecal ligation puncture group (group CLP), Sham and rosiglitazone group (group Sham+ ROSI), sham group (group Sham). Six rats were sacrificed at 0, 6, 12, 24 h after CLP respectively in each group. The general status of rats was observed and H&E staining was applied to detect renal pathological histology changes. Myeloperoxidase(MPO) was measured in kidney tissues. Expression of the ligand of peroxisome proliferators-activated receptor γ (PPARγ), and B-cell lymphoma-2(Bcl-2) were also determined by Western blot analysis. RESULTS: The increased MPO activity of group CLP+ ROSI was significantly lower than that of group CLP at 12 h and 24 h[(4.07±0.16)vs(4.62±0.08); P12=0.01, P24=0.006]. The MPO activity of group CLP was significantly higher than that of group Sham at 6, 12 and 24 h[(4.62±0.08)vs(1.30±0.06); P6=0.00, P12=0.00, P24=0.00]. The MPO activity of group CLP+ ROSI was significantly higher than that of group Sham+ ROSI at 6, 12 and 24 h[(4.07±0.16)vs(1.27±0.06); P6=0.00, P12=0.00, P24=0.00]. Compared with group Sham and group Sham+ ROSI, in rats of group CLP and group CLP+ ROSI the symptoms of sepsis and organ dysfunction were observed. The symptoms of sepsis and organ dysfunction of group CLP+ ROSI were significantly reduced than that of group CLP. Expression of PPARγ and Bcl-2 were determined by Western blot analysis, the expression of PPARγ and Bcl-2 of group CLP+ ROSI was significantly lower than that of group CLP. CONCLUSION:Rosiglitazone, as the ligand of PPARγ, reduced the inflammatory response in sepsis, decreased the apoptosis of kidney cell, reduced kidney injury, and protected acute kidney injury in rats of sepsis.