Literature DB >> 2752402

Pig brain homogenates contain smooth muscle myosin and cytoplasmic myosin isoforms.

U Gröschel-Stewart1, E Magel, E Paul, A C Neidlinger.   

Abstract

Three myosin isoforms, two of smooth muscle and one of cytoplasmic origin, were found in porcine brain by Western blotting analysis with antibodies specific for smooth and cytoplasmic myosins. The smooth muscle isoforms comprise at least 30% of the total myosin present. Brain tissue is therefore not a suitable source for the isolation of pure cytoplasmic contractile proteins.

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Year:  1989        PMID： 2752402     DOI： 10.1007/bf00221643

Source DB:  PubMed          Journal:  Cell Tissue Res        ISSN： 0302-766X            Impact factor:   5.249


  18 in total

1.  A simple ultraviolet spectrophotometric method for the determination of protein.

Authors:  W J WADDELL
Journal:  J Lab Clin Med       Date:  1956-08

2.  Production of specific antibodies to contractile proteins and their use in immunofluorescence microscopy. III. Antiobody against human uterine smooth muscle myosin.

Authors:  B Burkl; C Mahlmeister; U Gröschel-Stewart; J Chamley-Campbell; G Campbell
Journal:  Histochemistry       Date:  1979-04-03

3.  Actomyosin-like protein isolated from mammalian brain.

Authors:  S Puszkin; S Berl; E Puszkin; D D Clarke
Journal:  Science       Date:  1968-07-12       Impact factor: 47.728

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Expression of smooth muscle and nonmuscle myosin heavy chains in cultured vascular smooth muscle cells.

Authors:  A S Rovner; R A Murphy; G K Owens
Journal:  J Biol Chem       Date:  1986-11-05       Impact factor: 5.157

6.  Immunohistochemical studies with antibodies to myosins from the cytoplasm and membrane fraction of human blood platelets.

Authors:  U Gröschel-Stewart; C Rakousky; R Franke; I Peleg; I Kahane; A Eldor; A Muhlrad
Journal:  Cell Tissue Res       Date:  1985       Impact factor: 5.249

7.  Thymus myosin. Isolation and characterization of myosin from calf thymus and thymic lymphocytes, and studies on the effect of phosphorylation of its Mr = 20,000 light chain.

Authors:  J M Scholey; R C Smith; D Drenckhahn; U Groschel-Stewart; J Kendrick-Jones
Journal:  J Biol Chem       Date:  1982-07-10       Impact factor: 5.157

8.  Site-specific phosphorylation of brain myosin light chains by calcium-dependent and calcium-independent myosin kinases.

Authors:  S Matsumura; N Murakami; S Yasuda; A Kumon
Journal:  Biochem Biophys Res Commun       Date:  1982-12-15       Impact factor: 3.575

Review 9.  Immunochemistry of cytoplasmic contractile proteins.

Authors:  U Gröschel-Stewart
Journal:  Int Rev Cytol       Date:  1980

10.  Immunization, isolation of immunoglobulins, estimation of antibody titre.

Authors:  N Harboe; A Ingild
Journal:  Scand J Immunol Suppl       Date:  1973
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  9 in total

1.  Embryonic chicken gizzard: immunolocalization of collagen and smooth muscle myosin.

Authors:  E R Paul; T L Vo; A Meyer; U Gröschel-Stewart
Journal:  Cell Tissue Res       Date:  1992-11       Impact factor: 5.249

2.  Hexamethylenebisacetamide selectively inhibits the proliferation of human and rat vascular smooth-muscle cells.

Authors:  D J Grainger; T R Hesketh; P L Weissberg; J C Metcalfe
Journal:  Biochem J       Date:  1992-04-15       Impact factor: 3.857

3.  Neural and smooth muscle development in the chicken gizzard.

Authors:  Astrid Zimmermann; Anke Haina; Ute Gröschel-Stewart
Journal:  Rouxs Arch Dev Biol       Date:  1995-03

4.  Embryonic chicken gizzard: expression of the smooth muscle regulatory proteins caldesmon and myosin light chain kinase.

Authors:  E R Paul; P K Ngai; M P Walsh; U Gröschel-Stewart
Journal:  Cell Tissue Res       Date:  1995-02       Impact factor: 5.249

5.  Embryonic chicken gizzard: smooth muscle and non-muscle myosin isoforms.

Authors:  E R Paul; A L Christian; R Franke; U Gröschel-Stewart
Journal:  Cell Tissue Res       Date:  1994-05       Impact factor: 5.249

6.  Basal cells of H-Dunning tumor are myoepithelial cells. A comparative immunohistochemical and ultrastructural study with male accessory sex glands and mammary gland.

Authors:  G Aumüller; U Gröschel-Stewart; M Altmannsberger; H G Mannherz; M Steinhoff
Journal:  Histochemistry       Date:  1991

7.  Transforming growth factor beta decreases the rate of proliferation of rat vascular smooth muscle cells by extending the G2 phase of the cell cycle and delays the rise in cyclic AMP before entry into M phase.

Authors:  D J Grainger; P R Kemp; C M Witchell; P L Weissberg; J C Metcalfe
Journal:  Biochem J       Date:  1994-04-01       Impact factor: 3.857

8.  Expression of myosin heavy and light chains changes during pregnancy in the rat uterus.

Authors:  I Morano; G Erb; B Sogl
Journal:  Pflugers Arch       Date:  1993-06       Impact factor: 3.657

9.  A large accumulation of non-muscle myosin occurs at first entry into M phase in rat vascular smooth-muscle cells.

Authors:  D J Grainger; T R Hesketh; J C Metcalfe; P L Weissberg
Journal:  Biochem J       Date:  1991-07-01       Impact factor: 3.857
  9 in total

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