Ran Yu1, Yumei Ding1, Lijuan Zhu1, Yinying Qu2, Chenguang Zhang1, Lin Liu3, Lili Chen4. 1. Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022 Wuhan, China. 2. Dalian Stomatological Hospital of Dalian Medical University, 116021 Dalian, China. 3. Dalian Stomatological Hospital of Dalian Medical University, 116021 Dalian, China. Electronic address: 305802104@qq.com. 4. Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022 Wuhan, China. Electronic address: chenlili@whuh.com.
Abstract
OBJECTIVE: Wounds are common in the oral cavity. During wound healing, several cytokines are released, which are probably helpful in providing wound debridement, removal of damaged tissues and microbes. Most of the target cells of IL-22 are epithelial cells, which play an important role in mucosa immunity. DESIGN: The function of IL-22 in oral diseases is not well understood. We investigated the expression level of IL-22, collagen I and p-stat3 (Tyr705) via a mice tongue wound model in vivo and detected the effect of IL-22 on the expression of MMP-1, type I collagen and p-stat3 in keratinocytes. RESULTS: IL-22 and p-stat3 were associated with wound healing, and STAT3 was activated when the keratinocytes or the tongue tissue were stimulated by IL-22. In addition, IL-22 could mediate gene expression involved in wounds involving keratinocytes, such as type I collagen and MMP-1, which may contribute to scarless healing. CONCLUSION: Our study suggests that IL-22 mediates wound healing via STAT3 in keratinocytes. This study reveals a new role for IL-22 in mediating wound healing.
OBJECTIVE: Wounds are common in the oral cavity. During wound healing, several cytokines are released, which are probably helpful in providing wound debridement, removal of damaged tissues and microbes. Most of the target cells of IL-22 are epithelial cells, which play an important role in mucosa immunity. DESIGN: The function of IL-22 in oral diseases is not well understood. We investigated the expression level of IL-22, collagen I and p-stat3 (Tyr705) via a mice tongue wound model in vivo and detected the effect of IL-22 on the expression of MMP-1, type I collagen and p-stat3 in keratinocytes. RESULTS:IL-22 and p-stat3 were associated with wound healing, and STAT3 was activated when the keratinocytes or the tongue tissue were stimulated by IL-22. In addition, IL-22 could mediate gene expression involved in wounds involving keratinocytes, such as type I collagen and MMP-1, which may contribute to scarless healing. CONCLUSION: Our study suggests that IL-22 mediates wound healing via STAT3 in keratinocytes. This study reveals a new role for IL-22 in mediating wound healing.