Lijuan Ding1, Shudong Wang2, Xiaozhang Qu3, Jiafeng Wang4. 1. Department of Radiation Oncology, The First Hospital of Jilin University, Changchun 130021, China. 2. Department of Cardiology, The First Hospital of Jilin University, Changchun 130021, China. 3. Department of Endocrinology, The Second Part of the First Hospital, Jilin University, Changchun 130021, China. 4. Department of Endodontics, School and Hospital of Stomatology, Jilin University, Changchun 130021, China. Electronic address: jiafeng_wang88@126.com.
Abstract
PURPOSE: Tanshinone IIA (TanIIA), which is derived from the roots of Salvia miltiorrhiza (Danshen), has multiple pharmacological activities. However, the radiosensitivezing activity of TanIIA in oral cancer cells has not been studied extensively. The purpose of this study is to investigate the radiosensitizing effects of TanIIA in human oral squamous cell carcinoma SCC090 and examined the underlying mechanisms. METHODS: Clonogenic assay was used to investigate the radiosensitizing effect of TanIIA on SCC090. And then, apoptosis and reactive oxygen species (ROS) induced by the combination of TanIIA with radiation were analyzed by Flow cytometry. Finally, autophagy was detected by monodansylcadervarine (MDC) staining. RESULTS: TanIIA could significantly sensitize SCC090 to radiation. Meanwhile, an increase ROS generation after exposed to the combination treatment was found. In addition, the protein levels of Beclin 1, Atg5 and LC3-II, three important proteins involved in autophagy were increased in cells. CONCLUSIONS: TanIIA exerted a strong radiosensitizing effect on SCC090 comparing with the simple drug or single radiation treatment, which was due to an enhanced ROS generation and autophagy.
PURPOSE: Tanshinone IIA (TanIIA), which is derived from the roots of Salvia miltiorrhiza (Danshen), has multiple pharmacological activities. However, the radiosensitivezing activity of TanIIA in oral cancer cells has not been studied extensively. The purpose of this study is to investigate the radiosensitizing effects of TanIIA in humanoral squamous cell carcinoma SCC090 and examined the underlying mechanisms. METHODS: Clonogenic assay was used to investigate the radiosensitizing effect of TanIIA on SCC090. And then, apoptosis and reactive oxygen species (ROS) induced by the combination of TanIIA with radiation were analyzed by Flow cytometry. Finally, autophagy was detected by monodansylcadervarine (MDC) staining. RESULTS: TanIIA could significantly sensitize SCC090 to radiation. Meanwhile, an increase ROS generation after exposed to the combination treatment was found. In addition, the protein levels of Beclin 1, Atg5 and LC3-II, three important proteins involved in autophagy were increased in cells. CONCLUSIONS: TanIIA exerted a strong radiosensitizing effect on SCC090 comparing with the simple drug or single radiation treatment, which was due to an enhanced ROS generation and autophagy.