Differential effects of amino acid analogs on growth and heterocyst differentiation in two nitrogen-fixing blue-green algae.

Effects of a few amino acid analogs on growth and heterocyst differentiation have been studied in two nitrogen-fixing species ofAnabaena. All the analogs except α-methyl-DL-aspartic acid inhibited growth. Exposure ofAnabaena doliolum, toDL-5-fluorotryptophan andDL-p-fluorophenylalanine caused pronounced fragmentation of filaments into single cells. At low concentrations (0.01 mM), α-methyl-DL-aspartic acid stimulated growth of the strain ofA. doliolum as well as the strain of the second (unidentified)Anabaena species. Ethionine,DL-p-fluorophenylalanine,DL-5-fluorotryptophan, and canavanine blocked heterocyst differentiation, whereas α-methyl-DL-aspartic acid, α-methyl-DL-methionine,N-o-nitrophenylsulfenyl-L-tryptophan, norleucine, andS-2-aminoethyl-L-cysteine did not show any significant effect. Treatment with 7-azatryptophan,DL-β-hydroxynorvaline,L-methionine-DL-sulfoximine,L-methionine sulfone, and β-2-thienyl-DL-alanine led to a twofold increase in heterocyst frequency. Possible modes of action of the analogs in growth inhibition and changes in heterocyst frequency are discussed.