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Literature DB >> 27519249

Phospholipase C catalyzed formation of sphingomyelin-(14)C from lecithin and N-((14)C)-oleoyl-sphingosine.

Abstract

Commercial preparations ofClostridium perfringens were incubated with phosphatidyl choline and N-1-((14)C) oleoylsphingosine. A radioactive product was formed which cochromatogramed with sphingomyelin standard in three different solvent systems. Several other phospholipases and phosphatases were unable to catalyze this reaction. Neither choline, phosphoryl choline, cytidine diphosphate choline nor p-nitrophenyl phosphoryl choline were active donors. Sphingomyelin was only slightly active as a phosphoryl choline donor.

Entities: Chemical Species

Year: 1975 PMID： 27519249 DOI： 10.1007/BF02532442

Source DB: PubMed Journal: Lipids ISSN： 0024-4201 Impact factor: 1.880

15 in total

1. OBSERVATIONS ON CEREBROSIDE METABOLISM IN VIVO.

Authors: J KANFER
Journal: J Biol Chem Date: 1965-02 Impact factor: 5.157

2. The enzymatic synthesis of sphingomyelin.

Authors: M SRIBNEY; E P KENNEDY
Journal: J Biol Chem Date: 1958-12 Impact factor: 5.157

3. The biochemistry of bacterial toxins: The lecithinase activity of Cl. welchii toxins.

Authors: M G Macfarlane; B C Knight
Journal: Biochem J Date: 1941-09 Impact factor: 3.857

4. Kinetics of incorporation of ( 32 P)phosphate into phospholipids of a SV 40 transformed mouse cell during logarithmic growth.

Authors: W D Marggraf; H Diringer; M A Koch; F A Anderer
Journal: Hoppe Seylers Z Physiol Chem Date: 1972-11

5. Evidence for a new biosynthetic pathway of sphingomyelin in SV 40 transformed mouse cells.

Authors: H Diringer; W D Marggraf; M A Koch; F A Anderer
Journal: Biochem Biophys Res Commun Date: 1972-06-28 Impact factor: 3.575

6. Biosynthesis of sphingomyelin. Transfer of phosphorylcholine from phosphatidylcholine to erythro-ceramide in a cell-free system.

Authors: H Diringer; M A Koch
Journal: Hoppe Seylers Z Physiol Chem Date: 1973-12

7. Alternative pathways in the biosynthesis of sphingomyelin and the role of phosphatidylcholine, CDPcholine and phosphorylcholine as precursors.

Authors: W D Marggraf; F A Anderer
Journal: Hoppe Seylers Z Physiol Chem Date: 1974-07

Phospholipase C catalyzed formation of sphingomyelin-(14)C from lecithin and N-((14)C)-oleoyl-sphingosine.

1. OBSERVATIONS ON CEREBROSIDE METABOLISM IN VIVO.

2. The enzymatic synthesis of sphingomyelin.

3. The biochemistry of bacterial toxins: The lecithinase activity of Cl. welchii toxins.

4. Kinetics of incorporation of ( 32 P)phosphate into phospholipids of a SV 40 transformed mouse cell during logarithmic growth.

5. Evidence for a new biosynthetic pathway of sphingomyelin in SV 40 transformed mouse cells.

6. Biosynthesis of sphingomyelin. Transfer of phosphorylcholine from phosphatidylcholine to erythro-ceramide in a cell-free system.

7. Alternative pathways in the biosynthesis of sphingomyelin and the role of phosphatidylcholine, CDPcholine and phosphorylcholine as precursors.

8. The enzymatic formation of sphingomyelin from ceramide and lecithin in mouse liver.

9. Complete purification and some properties of phospholipase C from Bacillus cereus.

10. Transphosphatidylation by phospholipase D.

1. Preparation of acylglycerols and phospholipids with the aid of lipolytic enzymes.