| Literature DB >> 27517396 |
Abstract
Some factors affecting determination of sialic acids by the Warren thiobarbituric acid method are reported. These are: (1) conditions of hydrolysis, (2) sample size, (3) use of trichloroacetic acid and (4) stability of thiobarbituric acid solutions. Use of the modifications described here results in more reliable values. In addition, an improved procedure for chromphore production is outlined. It should be noted that failure to observe the suggested precautions can lead to cumulative errors.Entities:
Year: 1979 PMID： 27517396 DOI： 10.1007/BF02938058
Source DB: PubMed Journal: Ir J Med Sci ISSN： 0021-1265 Impact factor: 1.568