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Literature DB >> 27517262

High-sensitivity radioimmunoassay for substance P.

R O'Connell¹, P Skrabanek¹, D Cannon¹, D Powell¹.

Abstract

Optimisation of a non-equilibrium radioimmunoassay for substance P is described. The assay has a sensitivity of 2 pg (1.5 fmole), sufficient for substance P determinations in unexcepted human plasma and other biological material. The specificity of antisera used was tested with closely related peptides, physalaemin and eledoisin. Crossreaction of 1 per cent for eledoisin and 0.1 per cent for physalaemin was found. The assay is easy to perform, has a precision of 5 per cent, and takes only 24 hours.

Entities: Gene Species

Year: 1976 PMID： 27517262 DOI： 10.1007/BF02938978

Source DB: PubMed Journal: Ir J Med Sci ISSN： 0021-1265 Impact factor: 1.568

19 in total

1. Subcellular distribution of substance P in bovine hypothalamus and substantia nigra.

Authors: M J Duffy; D Mulhall; D Powell
Journal: J Neurochem Date: 1975-09 Impact factor: 5.372

2. Substance P and spinal neurones.

Authors: J L Henry; K Krnjevíc; M E Morris
Journal: Can J Physiol Pharmacol Date: 1975-06 Impact factor: 2.273

3. [Central transmission of afferent impulses. III. Incidence and significance of the substance P in the dorsal roots of the spinal cord].

Authors: F LEMBECK
Journal: Naunyn Schmiedebergs Arch Exp Pathol Pharmakol Date: 1953

High-sensitivity radioimmunoassay for substance P.

1. Subcellular distribution of substance P in bovine hypothalamus and substantia nigra.

2. Substance P and spinal neurones.

3. [Central transmission of afferent impulses. III. Incidence and significance of the substance P in the dorsal roots of the spinal cord].

4. An unidentified depressor substance in certain tissue extracts.

5. Identification of the motoneuron-depolarizing peptide in bovine dorsal root as hypothalamic substance P.

6. The experimental generation of antibodies to alpha-melanocyte stimulating hormone and adrenocorticotropic hormone.

7. Isolation of a sialogogic peptide from bovine hypothalamic tissue and its characterization as substance P.

8. Synthesis of substance P.

9. Amino-acid sequence of substance P.

10. Localization of substance P-like immunoreactivity in mouse gut.

1. Plasma substance P in the irritable bowel syndrome.

2. Substance P in human adrenal medulla and in sympathetic tumours.