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Literature DB >> 27517258

A microculture technique in the evaluation of lymphocyte transformation by mitogens and specific antigens in humans.

Abstract

A micro method forin vitro transformation of lymphocytes (Penhaleet al., 1974) by mitogens and antigens is presented. Using a range of concentrations, satisfactory responses were obtained with small numbers of lymphocytes in the presence of the mitogens, phytohaemagglutinin-P, Concanavalin-A, Pokeweed mitogen and mumps, tetanus, tuberculin (Purified Protein Derivative) antigens. Diptheria toxoid and streptokinase/streptodornase antigens did not give satisfactory responses. The method is likely to be of particular use in studies in lymphopenic individuals.

Entities: Disease Species

Year: 1976 PMID： 27517258 DOI： 10.1007/BF02938974

Source DB: PubMed Journal: Ir J Med Sci ISSN： 0021-1265 Impact factor: 1.568

14 in total

1. PHA stimulation of separated human lymphocyte populations.

Authors: M R Potter; M Moore
Journal: Clin Exp Immunol Date: 1975-09 Impact factor: 4.330

2. The response to phytohemagglutinin or to concanavalin A as a probe for subpopulations of human peripheral blood lymphocytes.

Authors: A J Rawson; T C Huang
Journal: Cell Immunol Date: 1975-05 Impact factor: 4.868

3. Comparison of Candida-delayed hypersensitivity skin test size with lymphocyte transformation, migration inhibitory factor production and antibody titer.

Authors: D E Bice; M Lopez; H Rothschild; J Salvaggio
Journal: Int Arch Allergy Appl Immunol Date: 1974

9. A new micromethod for evaluating lymphocyte responses to phytohemagglutinin: quantitative analysis of the function of thymus-dependent cells.

Authors: B H Park; R A Good
Journal: Proc Natl Acad Sci U S A Date: 1972-02 Impact factor: 11.205

10. The effect of in vivo hydrocortisone on subpopulations of human lymphocytes.

Authors: A S Fauci; D C Dale
Journal: J Clin Invest Date: 1974-01 Impact factor: 14.808

1 in total

1. Cell mediated immunity to recall antigens in vivo and in vitro.

Authors: A M Hassett; R J Woods; I J Temperley; G M Mullins
Journal: Ir J Med Sci Date: 1977-06 Impact factor: 1.568

1 in total

A microculture technique in the evaluation of lymphocyte transformation by mitogens and specific antigens in humans.

1. PHA stimulation of separated human lymphocyte populations.

2. The response to phytohemagglutinin or to concanavalin A as a probe for subpopulations of human peripheral blood lymphocytes.

3. Comparison of Candida-delayed hypersensitivity skin test size with lymphocyte transformation, migration inhibitory factor production and antibody titer.

4. Response of human thymus-derived (T) and non-thymus-derived (B) lymphocytes to mitogenic stimulation in vitro.

5. Heterogeneity of "acquired" or common variable agammaglobulinemia.

6. A rapid micro-method for the phytohaemagglutinin-induced human lymphocyte transformation test.

7. Evaluation of methods for preparing pure lymphocyte suspensions from peripheral blood.

8. B- and T-cell markers on human lymphoblasts after stimulation with mitogen or antigens.

9. A new micromethod for evaluating lymphocyte responses to phytohemagglutinin: quantitative analysis of the function of thymus-dependent cells.

10. The effect of in vivo hydrocortisone on subpopulations of human lymphocytes.

1. Cell mediated immunity to recall antigens in vivo and in vitro.