Andrew M Zogby1, Sudarshan Dayanidhi2,3, Henry G Chambers2,4, Simon Schenk2,5, Richard L Lieber2,3,6. 1. School of Medicine, University of California, San Diego, California, USA. 2. Department of Orthopaedic Surgery, University of California, San Diego, California, USA. 3. Department of Veterans Affairs Medical Center, San Diego, California, USA. 4. Department of Orthopaedics, Rady Children's Hospital, San Diego, California, USA. 5. Biomedical Sciences Program, University of California, San Diego, California, USA. 6. Department of Bioengineering, University of California, San Diego, California, USA.
Abstract
INTRODUCTION: Children with cerebral palsy (CP) exhibit increased energy expenditure during movement, but whether this is due in part to decrements in skeletal muscle mitochondrial oxidative capacity is unknown. Accordingly, we compared fiber-type specific succinate dehydrogenase (SDH) activity in children with CP with typically developing (TD) children. METHODS: SDH activity and myofiber areas of type 1 and 2A fibers were measured in semitendinosus biopsies of both groups (n = 5/group). RESULTS: SDH activity was ∼35% higher in type 1 compared with type 2A fibers, but there were no differences between groups. Average myofiber area was 45% smaller in CP versus TD (P < 0.05), and type 2A fibers were 32% larger than type 1 fibers (P < 0.05) only in TD children. CONCLUSIONS: Fiber-type specific SDH activity is similar between TD children and children with CP. This suggests that increased energy expenditure in children with CP is not related to impaired mitochondrial oxidative capacity. Muscle Nerve, 2016 Muscle Nerve 55: 122-124, 2017.
INTRODUCTION:Children with cerebral palsy (CP) exhibit increased energy expenditure during movement, but whether this is due in part to decrements in skeletal muscle mitochondrial oxidative capacity is unknown. Accordingly, we compared fiber-type specific succinate dehydrogenase (SDH) activity in children with CP with typically developing (TD) children. METHODS:SDH activity and myofiber areas of type 1 and 2A fibers were measured in semitendinosus biopsies of both groups (n = 5/group). RESULTS:SDH activity was ∼35% higher in type 1 compared with type 2A fibers, but there were no differences between groups. Average myofiber area was 45% smaller in CP versus TD (P < 0.05), and type 2A fibers were 32% larger than type 1 fibers (P < 0.05) only in TD children. CONCLUSIONS: Fiber-type specific SDH activity is similar between TD children and children with CP. This suggests that increased energy expenditure in children with CP is not related to impaired mitochondrial oxidative capacity. Muscle Nerve, 2016 Muscle Nerve 55: 122-124, 2017.
Authors: Sudarshan Dayanidhi; Peter B Dykstra; Vera Lyubasyuk; Bryon R McKay; Henry G Chambers; Richard L Lieber Journal: J Orthop Res Date: 2015-04-10 Impact factor: 3.494
Authors: Yajuan Li; Yaohua Zhang; Qingsong Hu; Sergey D Egranov; Zhen Xing; Zhao Zhang; Ke Liang; Youqiong Ye; Yinghong Pan; Sujash S Chatterjee; Brandon Mistretta; Tina K Nguyen; David H Hawke; Preethi H Gunaratne; Mien-Chie Hung; Leng Han; Liuqing Yang; Chunru Lin Journal: Genome Med Date: 2021-08-28 Impact factor: 11.117