Literature DB >> 27514807

NMR Spectroscopy to Study MAP Kinase Binding to MAP Kinase Phosphatases.

Wolfgang Peti1,2, Rebecca Page3.   

Abstract

NMR spectroscopy and other solution methods are increasingly being used to obtain novel insights into the mechanisms by which MAPK regulatory proteins bind and direct the activity of MAPKs. Here, we describe how interactions between the MAPK p38α and its regulatory proteins are studied using NMR spectroscopy, isothermal titration calorimetry, and small angle X-ray scattering (SAXS).

Keywords:  Chemical shift perturbation (CSP); D2O; Dual specificity phosphatase; Isothermal titration calorimetry (ITC); Isotopically labeled growth media; Mitogen activated protein kinase (MAPK); Nuclear magnetic resonance (NMR) spectroscopy; Protein tyrosine phosphatases; Protein–protein interaction; Small angle X-ray scattering (SAXS); p38α

Mesh：

Substances：

Year:  2016        PMID： 27514807     DOI： 10.1007/978-1-4939-3746-2_11

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  8 in total

1.  Conformational Rigidity and Protein Dynamics at Distinct Timescales Regulate PTP1B Activity and Allostery.

Authors:  Meng S Choy; Yang Li; Luciana E S F Machado; Micha B A Kunze; Christopher R Connors; Xingyu Wei; Kresten Lindorff-Larsen; Rebecca Page; Wolfgang Peti
Journal:  Mol Cell       Date:  2017-02-16       Impact factor: 17.970

2.  The KIM-family protein-tyrosine phosphatases use distinct reversible oxidation intermediates: Intramolecular or intermolecular disulfide bond formation.

Authors:  Luciana E S F Machado; Tun-Li Shen; Rebecca Page; Wolfgang Peti
Journal:  J Biol Chem       Date:  2017-04-07       Impact factor: 5.157

3.  Cooperative dynamics across distinct structural elements regulate PTP1B activity.

Authors:  Kristiane R Torgeson; Michael W Clarkson; Ganesan Senthil Kumar; Rebecca Page; Wolfgang Peti
Journal:  J Biol Chem       Date:  2020-07-31       Impact factor: 5.157

4.  Structures of Dynamic Protein Complexes: Hybrid Techniques to Study MAP Kinase Complexes and the ESCRT System.

Authors:  Wolfgang Peti; Rebecca Page; Evzen Boura; Bartosz Różycki
Journal:  Methods Mol Biol       Date:  2018

5.  The interaction of p38 with its upstream kinase MKK6.

Authors:  Ganesan Senthil Kumar; Rebecca Page; Wolfgang Peti
Journal:  Protein Sci       Date:  2021-02-16       Impact factor: 6.725

6.  EDC3 phosphorylation regulates growth and invasion through controlling P-body formation and dynamics.

Authors:  Jeremiah J Bearss; Sathish Kr Padi; Neha Singh; Marina Cardo-Vila; Jin H Song; Ghassan Mouneimne; Nikita Fernandes; Yang Li; Matthew R Harter; Jaime Mc Gard; Anne E Cress; Wolfgang Peti; Andrew Dl Nelson; J Ross Buchan; Andrew S Kraft; Koichi Okumura
Journal:  EMBO Rep       Date:  2021-02-15       Impact factor: 8.807

7.  The catalytic activity of TCPTP is auto-regulated by its intrinsically disordered tail and activated by Integrin alpha-1.

Authors:  Jai Prakash Singh; Yang Li; Yi-Yun Chen; Shang-Te Danny Hsu; Rebecca Page; Wolfgang Peti; Tzu-Ching Meng
Journal:  Nat Commun       Date:  2022-01-10       Impact factor: 14.919

8.  The structure of the RCAN1:CN complex explains the inhibition of and substrate recruitment by calcineurin.

Authors:  Yang Li; Sarah R Sheftic; Simina Grigoriu; Charles D Schwieters; Rebecca Page; Wolfgang Peti
Journal:  Sci Adv       Date:  2020-07-01       Impact factor: 14.136
  8 in total

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