| Literature DB >> 27514802 |
Laura Spinelli1,2, Nicholas R Leslie3.
Abstract
PTEN is a one of the most frequently mutated tumor suppressors in human cancers. It is essential for regulating diverse biological processes and through its lipid phosphatase activity regulates the PI 3-Kinase signaling pathway. Sensitive phosphatase assays are employed to study the catalytic activity of PTEN against phospholipid substrates. Here we describe protocols to assay PTEN lipid phosphatase activity using either purified enzyme (purified PTEN lipid phosphatase assay) or PTEN immunopurified from tissues or cultured cells (cellular IP PTEN lipid phosphatase assay) against vesicles containing radiolabeled PIP3 substrate.Entities:
Keywords: Cancer; PI3-Kinase; PTEN; Phosphatase; Phosphate; Phosphoinositide; Tumor suppressor
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Year: 2016 PMID： 27514802 DOI： 10.1007/978-1-4939-3746-2_6
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745