Abraham Silva Carmona1, Hugo Mendieta Zerón2. 1. Hospital General de México, SSA, Mexico City, Mexico. 2. Maternal-Perinatal Hospital "Mónica Pretelini Sáenz" (HMPMPS), Health Institute of the State of Mexico (ISEM), Toluca, Mexico.
Abstract
BACKGROUND/AIM: Preeclampsia is a leading cause of maternal death in the developing world. Our aim was to quantify and compare messenger (mRNA) expression of nuclear factor-kappa beta (NF-κΒ) and superoxide dismutase (SOD) in control patients with preeclampsia and without preeclampsia with or without familial hereditary background. MATERIALS AND METHODS: Four groups of patients were formed depending on the presence or absence of preeclampsia and presence or absence of familial history for preeclampsia. NF-κΒ and SOD were measured in human placentas by real-time quantitative polymerase chain reaction. The 2-ΔΔct analysis method was used to measure the difference in the relative expression of the target genes in each group of patients. RESULTS: In NF-κΒ expression, there was an increase of 23.35% in the group of women with preeclampsia versus women with preeclampsia without familial history. Regarding SOD, there was a reduction of about 33.33% in the expression in women with preeclampsia with familial history versus women with preeclampsia without familial history. CONCLUSION: Familial presence of preeclampsia could predispose to altered expression in SOD and NF-κΒ.
BACKGROUND/AIM: Preeclampsia is a leading cause of maternal death in the developing world. Our aim was to quantify and compare messenger (mRNA) expression of nuclear factor-kappa beta (NF-κΒ) and superoxide dismutase (SOD) in control patients with preeclampsia and without preeclampsia with or without familial hereditary background. MATERIALS AND METHODS: Four groups of patients were formed depending on the presence or absence of preeclampsia and presence or absence of familial history for preeclampsia. NF-κΒ and SOD were measured in human placentas by real-time quantitative polymerase chain reaction. The 2-ΔΔct analysis method was used to measure the difference in the relative expression of the target genes in each group of patients. RESULTS: In NF-κΒ expression, there was an increase of 23.35% in the group of women with preeclampsia versus women with preeclampsia without familial history. Regarding SOD, there was a reduction of about 33.33% in the expression in women with preeclampsia with familial history versus women with preeclampsia without familial history. CONCLUSION: Familial presence of preeclampsia could predispose to altered expression in SOD and NF-κΒ.
Authors: Margarita L Martinez-Fierro; Idalia Garza-Veloz; Maria Eugenia Castañeda-Lopez; Dorothy Wasike; Claudia Castruita-De la Rosa; Iram Pablo Rodriguez-Sanchez; Ivan Delgado-Enciso; Jose Flores-Mendoza Journal: Int J Mol Sci Date: 2022-09-04 Impact factor: 6.208