Xiaofeng Sun1, Dajiang Xiao2, Ting Xu2, Yuan Yuan2. 1. Department of Stomatology, The Second People's Hospital of Wuxi, 68 Zhong Shan Road, Wuxi 214002, Jiangsu, PR China. 2. Department of Otolaryngology, The Second People's Hospital of Wuxi, 68 Zhong Shan Road, Wuxi 214002, Jiangsu, PR China.
Abstract
AIM: To investigate the role of miR-24-3p in tumorigenesis and chemosensitivity in head and neck squamous cell carcinoma (HNSCC). METHODS: Growth rate and colony formation assays were performed after transfection with miR-24-3p mimic and inhibitor in cultured SCC-15 cells, followed by a CellTiter-Glo® assay. Western blot and luciferase assays were performed to investigate the direct target of miR-24-3p. Xenograft mouse model was used to evaluate combinatorial effects of miR-24-3p inhibitor and 5-fluorouracil. RESULTS & CONCLUSION: Inhibition of miR-24-3p reduced cell proliferation, colony formation efficiency and reversed chemoresistance in HNSCC cells. CHD5 is the direct target of miR-24-3p which is required for the regulatory role of miR-24-3p in chemoresistance. miR-24-3p may represent a new therapeutic target for the improvement of clinical outcome in HNSCC.
AIM: To investigate the role of miR-24-3p in tumorigenesis and chemosensitivity in head and neck squamous cell carcinoma (HNSCC). METHODS: Growth rate and colony formation assays were performed after transfection with miR-24-3p mimic and inhibitor in cultured SCC-15 cells, followed by a CellTiter-Glo® assay. Western blot and luciferase assays were performed to investigate the direct target of miR-24-3p. Xenograft mouse model was used to evaluate combinatorial effects of miR-24-3p inhibitor and 5-fluorouracil. RESULTS & CONCLUSION: Inhibition of miR-24-3p reduced cell proliferation, colony formation efficiency and reversed chemoresistance in HNSCC cells. CHD5 is the direct target of miR-24-3p which is required for the regulatory role of miR-24-3p in chemoresistance. miR-24-3p may represent a new therapeutic target for the improvement of clinical outcome in HNSCC.
Authors: Ajaz A Bhat; Parvaiz Yousuf; Nissar A Wani; Arshi Rizwan; Shyam S Chauhan; Mushtaq A Siddiqi; Davide Bedognetti; Wael El-Rifai; Michael P Frenneaux; Surinder K Batra; Mohammad Haris; Muzafar A Macha Journal: Signal Transduct Target Ther Date: 2021-01-12