Claudia Pouliot-Bérubé1,2, Karine Zaniolo3, Sylvain L Guérin3,4, Roxane Pouliot1,2. 1. Centre LOEX de l'Université Laval, Génie Tissulaire et Régénération, Centre de Recherche FRQS du CHU de Québec, Axe Médecine Régénératrice, Québec, QC, Canada. 2. Faculté de Pharmacie, Université Laval, Québec, QC, Canada. 3. Centre Universitaire d'Ophtalmologie-Recherche, Centre de Recherche FRQS du CHU de Québec, Axe Médecine Régénératrice, Québec, QC, Canada. 4. Département d'Ophtalmologie, Université Laval, Québec, QC, Canada.
Abstract
AIM: Psoriasis is a chronic inflammatory skin disease. To study its complex etiology, a psoriatic skin substitute model supplemented with a cytokine cocktail has been used. MATERIALS & METHODS: Reconstructed psoriatic skin substitutes were supplemented with a cocktail of four cytokines: TNF-α, IL-1α, IL-6 and IL-17A, to monitor their impact on gene expression by DNA microarray. RESULTS: Gene profiling analyses identified several deregulated genes reported as being also deregulated in psoriasis skin in vivo (S100A12, IL-8, DEFB4A and KYNU). The expression of those genes was dramatically increased compared with basal levels of controls (p < 0.005 to < 0.05). CONCLUSION: Psoriatic substitutes supplemented with a cocktail of TNF-α, IL-1α, IL-6 and IL-17A showed similar transcriptome alterations to those found in psoriasis.
AIM: Psoriasis is a chronic inflammatory skin disease. To study its complex etiology, a psoriatic skin substitute model supplemented with a cytokine cocktail has been used. MATERIALS & METHODS: Reconstructed psoriatic skin substitutes were supplemented with a cocktail of four cytokines: TNF-α, IL-1α, IL-6 and IL-17A, to monitor their impact on gene expression by DNA microarray. RESULTS: Gene profiling analyses identified several deregulated genes reported as being also deregulated in psoriasis skin in vivo (S100A12, IL-8, DEFB4A and KYNU). The expression of those genes was dramatically increased compared with basal levels of controls (p < 0.005 to < 0.05). CONCLUSION: Psoriatic substitutes supplemented with a cocktail of TNF-α, IL-1α, IL-6 and IL-17A showed similar transcriptome alterations to those found in psoriasis.
Authors: Jos P H Smits; Hanna Niehues; Gijs Rikken; Ivonne M J J van Vlijmen-Willems; Guillaume W H J F van de Zande; Patrick L J M Zeeuwen; Joost Schalkwijk; Ellen H van den Bogaard Journal: Sci Rep Date: 2017-09-19 Impact factor: 4.379
Authors: Roudin Sarama; Priya K Matharu; Yousef Abduldaiem; Mab P Corrêa; Cristiane D Gil; Karin V Greco Journal: Front Bioeng Biotechnol Date: 2022-02-21