| Literature DB >> 27511165 |
Araceli Fominaya1, Yolanda Loarce2, Juan M González2, Esther Ferrer2.
Abstract
Tyramide signal amplification (TSA) fluorescence in situ hybridization (FISH) has been shown as a valuable molecular tool for visualizing specific amplified DNA sequences in chromosome preparations. This chapter describes how to perform TSA-FISH, paying special interest to its two critical steps: probe generation and metaphase plate generation. The potential of physically mapping 12S-globulin sequences by TSA-FISH as a means of identifying homeology among chromosome regions of Avena species was tested and is discussed.Entities:
Keywords: Avena; Metaphase chromosomes; Obtaining probes; Physical mapping; TSA-FISH
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Year: 2016 PMID： 27511165 DOI： 10.1007/978-1-4939-3622-9_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745