Justyna Podolak-Popinigis1,2,3, Anna Ronowicz1,2,3, Monika Dmochowska1,2,3, Agnieszka Jakubiak1,2,3, Paweł Sachadyn1,2,3. 1. Department of Molecular Biotechnology & Microbiology, Gdańsk University of Technology, Gdańsk, Poland. 2. Department of Biology & Pharmaceutical Botany, Medical University of Gdańsk, Gdańsk, Poland. 3. Tri-City Academic Laboratory Animal Centre - Research & Services Centre, Medical University of Gdańsk, Gdańsk, Poland.
Abstract
AIM: Fetal skin is known to heal without scarring. In mice, the phenomenon is observed until the 16-17 day of gestation - the day of transition from scarless to normal healing. The study aims to identify key methylome and transcriptome changes following the transition. MATERIALS & METHODS: Methylome and transcriptome profiles were analyzed in murine dorsal skin using microarray approach. RESULTS & CONCLUSION: The genes associated with inflammatory response and hyaluronate degradation showed increased DNA methylation before the transition, while those involved in embryonic morphogenesis, neuron differentiation and synapse functions did so after. A number of the methylome alterations were retained until adulthood and correlated with gene expression, while the functional associations imply that scarless healing depends on epigenetic regulation.
AIM: Fetal skin is known to heal without scarring. In mice, the phenomenon is observed until the 16-17 day of gestation - the day of transition from scarless to normal healing. The study aims to identify key methylome and transcriptome changes following the transition. MATERIALS & METHODS: Methylome and transcriptome profiles were analyzed in murine dorsal skin using microarray approach. RESULTS & CONCLUSION: The genes associated with inflammatory response and hyaluronate degradation showed increased DNA methylation before the transition, while those involved in embryonic morphogenesis, neuron differentiation and synapse functions did so after. A number of the methylome alterations were retained until adulthood and correlated with gene expression, while the functional associations imply that scarless healing depends on epigenetic regulation.
Authors: Paweł Sosnowski; Piotr Sass; Paulina Słonimska; Rafał Płatek; Jolanta Kamińska; Jakub Baczyński Keller; Piotr Mucha; Grażyna Peszyńska-Sularz; Artur Czupryn; Michał Pikuła; Arkadiusz Piotrowski; Łukasz Janus; Sylwia Rodziewicz-Motowidło; Piotr Skowron; Paweł Sachadyn Journal: Pharmaceuticals (Basel) Date: 2022-05-16