| Literature DB >> 27508873 |
Craig Porter1, David N Herndon2, Maria Chondronikola3, Tony Chao3, Palam Annamalai4, Nisha Bhattarai2, Manish K Saraf2, Karel D Capek2, Paul T Reidy5, Alexes C Daquinag6, Mikhail G Kolonin6, Blake B Rasmussen7, Elisabet Borsheim8, Tracy Toliver-Kinsky9, Labros S Sidossis2.
Abstract
Brown adipose tissue (BAT) plays an important role in mammalian thermoregulation. The component of BAT mitochondria that permits this function is the inner membrane carrier protein uncoupling protein 1 (UCP1). To the best of our knowledge, no studies have directly quantified UCP1 function in human BAT. Further, whether human and rodent BAT have comparable thermogenic function remains unknown. We employed high-resolution respirometry to determine the respiratory capacity, coupling control, and, most importantly, UCP1 function of human supraclavicular BAT and rodent interscapular BAT. Human BAT was sensitive to the purine nucleotide GDP, providing the first direct evidence that human BAT mitochondria have thermogenically functional UCP1. Further, our data demonstrate that human and rodent BAT have similar UCP1 function per mitochondrion. These data indicate that human and rodent BAT are qualitatively similar in terms of UCP1 function.Entities:
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Year: 2016 PMID： 27508873 PMCID： PMC5201422 DOI： 10.1016/j.cmet.2016.07.004
Source DB: PubMed Journal: Cell Metab ISSN： 1550-4131 Impact factor: 27.287