Ting Wang1, Shufeng Li2, Yun Yang2, Kaining Zhang2, Shixiao Dong3, Xiuhua Wang4, Xinguang Liu5, Yanjun Ren2, Ming Zhang2, Xinfeng Yan2, Jianmin Li6, Lei Zhang2. 1. Department of Obstetrics and Gynecology, Shandong Provincial Qianfoshan Hospital, Shandong University Jinan 250014, P.R. China. 2. Department of Orthopedics, Shandong Provincial Qianfoshan Hospital, Shandong University Jinan 250014, P.R. China. 3. Intensive Care Unit, Qilu Hospital, Shandong University Jinan 250012, P.R. China. 4. Department of Rheumatology, Shandong Provincial Qianfoshan Hospital, Shandong University Jinan 250014, P.R. China. 5. Department of Hematology, Qilu Hospital, Shandong University Jinan 250012, P.R. China. 6. Department of Orthopedics, Qilu Hospital, Shandong University Jinan 250012, P.R. China.
Abstract
OBJECTIVES: The present study is to investigate the profiles of Th17, Th1 and Treg cells in bone marrow of patients with rheumatoid arthritis (RA). METHODS: Flow cytometry was used to analyze the frequencies of Th17, Th1 and Treg cells in paired peripheral blood and bone marrow of 26 RA patients and 11 osteoarthritis (OA) patients, as well as 10 healthy controls. In addition, the disease activity was analyzed by the 28-joint disease activity score (DAS28). RESULTS: The frequencies of Th17 and Th1 cells were significantly elevated in bone marrow of RA patients. Importantly, Th17 and Th1 cells were significantly elevated in bone marrow compared with the matched peripheral blood from RA patients. However, Treg cells were significantly decreased in bone marrow of RA patients compared with the matched peripheral blood of RA patients and bone marrow of osteoarthritis patients and healthy controls. Moreover, the frequencies of tumor necrosis factor-α-producing T cells were significantly elevated in bone marrow from RA patients. Additionally, Th17 and Th1 cells in bone marrow were positively correlated with DAS28, while Treg cells were negatively correlated with DAS28. CONCLUSIONS: The present study demonstrates that Th17 and Th1 cells are markedly increased in bone marrow from RA patients. By contrast, Treg cells are significantly decreased in bone marrow from RA patients. These results suggest that local abnormality of Th17, Th1 and Treg cells in bone marrow of RA patients may contribute to bone destruction in skeletal system.
OBJECTIVES: The present study is to investigate the profiles of Th17, Th1 and Treg cells in bone marrow of patients with rheumatoid arthritis (RA). METHODS: Flow cytometry was used to analyze the frequencies of Th17, Th1 and Treg cells in paired peripheral blood and bone marrow of 26 RApatients and 11 osteoarthritis (OA) patients, as well as 10 healthy controls. In addition, the disease activity was analyzed by the 28-joint disease activity score (DAS28). RESULTS: The frequencies of Th17 and Th1 cells were significantly elevated in bone marrow of RApatients. Importantly, Th17 and Th1 cells were significantly elevated in bone marrow compared with the matched peripheral blood from RApatients. However, Treg cells were significantly decreased in bone marrow of RApatients compared with the matched peripheral blood of RApatients and bone marrow of osteoarthritispatients and healthy controls. Moreover, the frequencies of tumor necrosis factor-α-producing T cells were significantly elevated in bone marrow from RApatients. Additionally, Th17 and Th1 cells in bone marrow were positively correlated with DAS28, while Treg cells were negatively correlated with DAS28. CONCLUSIONS: The present study demonstrates that Th17 and Th1 cells are markedly increased in bone marrow from RApatients. By contrast, Treg cells are significantly decreased in bone marrow from RApatients. These results suggest that local abnormality of Th17, Th1 and Treg cells in bone marrow of RApatients may contribute to bone destruction in skeletal system.
Entities:
Keywords:
Rheumatoid arthritis; T helper 1 cells; T helper 17 cells; bone marrow; regulatory T cells
Authors: Jan Piet van Hamburg; Odilia B J Corneth; Sandra M J Paulissen; Nadine Davelaar; Patrick S Asmawidjaja; Adriana M C Mus; Erik Lubberts Journal: Ann Rheum Dis Date: 2013-01-17 Impact factor: 19.103
Authors: Hong Nie; Yingxia Zheng; Runsheng Li; Taylor B Guo; Dongyi He; Lei Fang; Xuebin Liu; Lianbo Xiao; Xi Chen; Bing Wan; Y Eugene Chin; Jingwu Z Zhang Journal: Nat Med Date: 2013-02-10 Impact factor: 53.440