Mahalakshmi Krishnan1, Padma Krishnan2, S C Chandrasekaran3. 1. Professor and Head, Department of Microbiology/Director, Research Lab for Oral-Systemic Health, Sree Balaji Dental College and Hospital, BIHER , Velachery - Tambaram Road, Chennai, India . 2. Assistant Professor, Department of Microbiology, Dr. ALM PGIBMS, University of Madras , Chennai, India . 3. Professor and Head, Department of Periodontics and Implantology, Sree Balaji Dental College and Hospital, BIHER , Velachery - Tambaram Road, Chennai, India .
Abstract
INTRODUCTION: Published literature till date reveals a high prevalence of Porphyromonas gingivalis fimA type I genotype among healthy subjects. Quite a few studies have reported its prevalence also in periodontitis patients. Nevertheless incidence of this genotype in gingivitis is lacking in adult population. AIM: The present study was chosen to detect P. gingivalis fimA type I genotype among chronic gingivitis patients. MATERIALS AND METHODS: A total of 46 subgingival plaque samples collected from chronic marginal gingivitis (n=23) and chronic periodontitis subjects (control group) (n=23) were subjected to Real-Time Polymerase Chain Reaction to detect the P. gingivalis fimA type I gene. Statistical analysis was performed using chi-square test. RESULTS: Prevalence of P. gingivalis fimA type I gene among chronic periodontitis and chronic gingivitis patients were 8.7% and 30.4% respectively. P. gingivalis fimA type I genotype prevalence was found to be statistically insignificant between the two study groups (p=0.135). CONCLUSION: The avirulent P. gingivalis fimA type I genotype, occurred in high prevalence among chronic gingivitis patients, while its presence was low in chronic periodontitis patients. Presence of this avirulent genotype in chronic marginal gingivitis signifies its reversible condition.
INTRODUCTION: Published literature till date reveals a high prevalence of Porphyromonas gingivalis fimA type I genotype among healthy subjects. Quite a few studies have reported its prevalence also in periodontitispatients. Nevertheless incidence of this genotype in gingivitis is lacking in adult population. AIM: The present study was chosen to detect P. gingivalis fimA type I genotype among chronic gingivitispatients. MATERIALS AND METHODS: A total of 46 subgingival plaque samples collected from chronic marginal gingivitis (n=23) and chronic periodontitis subjects (control group) (n=23) were subjected to Real-Time Polymerase Chain Reaction to detect the P. gingivalis fimA type I gene. Statistical analysis was performed using chi-square test. RESULTS: Prevalence of P. gingivalis fimA type I gene among chronic periodontitis and chronic gingivitispatients were 8.7% and 30.4% respectively. P. gingivalis fimA type I genotype prevalence was found to be statistically insignificant between the two study groups (p=0.135). CONCLUSION: The avirulent P. gingivalis fimA type I genotype, occurred in high prevalence among chronic gingivitispatients, while its presence was low in chronic periodontitispatients. Presence of this avirulent genotype in chronic marginal gingivitis signifies its reversible condition.
Entities:
Keywords:
Fimbriae; P. gingivalis; Periodontitis; Polymerase chain reaction
Authors: Juan Manuel Morillo; Laura Lau; Mariano Sanz; David Herrera; Conchita Martín; Augusto Silva Journal: J Clin Periodontol Date: 2004-12 Impact factor: 8.728
Authors: Khalil Boutaga; Arie Jan van Winkelhoff; Christina M J E Vandenbroucke-Grauls; Paul H M Savelkoul Journal: J Clin Periodontol Date: 2006-06 Impact factor: 8.728