Flávia K Tobaldini-Valerio1,2,3,4, Patricia S Bonfim-Mendonça1,2,3,4, Helen C Rosseto1,2,3,4, Marcos L Bruschi1,2,3,4, Mariana Henriques1,2,3,4, Melyssa Negri1,2,3,4, Sonia Silva1,2,3,4, Terezinha Ie Svidzinski1,2,3,4. 1. Laboratory of Medical Mycology, Department of Clinical Analysis & Biomedicine, Universidade Estadual de Maringá, Maringá, PR, Brazil. 2. CEB - Centre of Biological Engineering, Universidade do Minho, Braga, Portugal. 3. CAPES Foundation, Ministry of Education of Brazil, Brasilia - DF 70.040-020, Brazil. 4. Laboratory of Research & Development of Drug Delivery Systems, Department of Pharmacy, Universidade Estadual de Maringá, Maringá, PR, Brazil.
Abstract
AIM: To evaluate the effect of propolis against Candida species planktonic cells and its counterpart's biofilms. MATERIALS & METHODS: The MIC values, time-kill curves and filamentation form inhibition were determined in Candida planktonic cells. The effect of propolis on Candida biofilms was assessed through quantification of CFUs. RESULTS: MIC values, ranging from 220 to 880 µg/ml, demonstrated higher efficiency on C. albicans and C. parapsilosis than on C. tropicalis cells. In addition, propolis was able to prevent Candida species biofilm's formation and eradicate their mature biofilms, coupled with a significant reduction on C. tropicalis and C. albicans filamentation. CONCLUSION: Propolis is an inhibitor of Candida virulence factors and represents an innovative alternative to fight candidiasis.
AIM: To evaluate the effect of propolis against Candida species planktonic cells and its counterpart's biofilms. MATERIALS & METHODS: The MIC values, time-kill curves and filamentation form inhibition were determined in Candida planktonic cells. The effect of propolis on Candida biofilms was assessed through quantification of CFUs. RESULTS: MIC values, ranging from 220 to 880 µg/ml, demonstrated higher efficiency on C. albicans and C. parapsilosis than on C. tropicalis cells. In addition, propolis was able to prevent Candida species biofilm's formation and eradicate their mature biofilms, coupled with a significant reduction on C. tropicalis and C. albicans filamentation. CONCLUSION: Propolis is an inhibitor of Candida virulence factors and represents an innovative alternative to fight candidiasis.
Authors: Flavia F Veiga; Marina C Gadelha; Marielen R T da Silva; Maiara I Costa; Brenda Kischkel; Lidiane V de Castro-Hoshino; Francielle Sato; Mauro L Baesso; Morgana F Voidaleski; Vanessa Vasconcellos-Pontello; Vânia A Vicente; Marcos L Bruschi; Melyssa Negri; Terezinha I E Svidzinski Journal: Front Microbiol Date: 2018-04-25 Impact factor: 5.640
Authors: Jacqueline Teixeira da Silva; Matheus Gabriel de Oliveira; José Realino de Paula; Suzana Ferreira Alves; Flavio Pellegrini; Andre Correa Amaral Journal: Indian J Microbiol Date: 2021-06-16
Authors: Amara J Orth; Emma H Curran; Eric J Haas; Andrew C Kraemer; Audrey M Anderson; Nicholas J Mason; Carol A Fassbinder-Orth Journal: Insects Date: 2022-02-28 Impact factor: 2.769